UniProt Accession

 CBF1_YEAST; P17106; D6VWN1; P17623; Q6Q5N1 

Genbank Protein ID

 M33620; X52137; M34070; L47993; Z49560; AY557919; BK006943 

Genbank Nucleotide ID

 AAA34490.1; CAA36382.1; AAA34524.1; AAB39286.1; CAA89588.1; AAS56245.1; DAA08847.1 

Protein Name

 Centromere-binding protein 1 

Protein Synonyms/Alias

 CBP-1; Centromere promoter factor 1; Centromere-binding factor 1 

Gene Name

 CBF1 

Gene Synonyms/Alias

 CEP1; CP1; CPF1; YJR060W; J1730 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
271	AEYIQKLKETDEANI	ubiquitination	[1]
280	TDEANIEKWTLQKLL	acetylation	[2]
311	EELGNAYKEIEYMKR	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Required for chromosome stability and methionine prototrophy. It is involved in chromosomal segregation. Binds to a highly conserved DNA sequence (5'-RTCACRTG-3'), called CDEI, found in centromeres and in several promoters. DNA-binding activity is enhanced by MET28. Required as an auxiliary factor for transcriptional activation of sulfur metabolism together with MET4 and MET28. 

Sequence Annotation

 DOMAIN 222 270 bHLH.
 MOD_RES 45 45 Phosphoserine; by ATM or ATR.
 MOD_RES 48 48 Phosphoserine.
 MOD_RES 84 84 Phosphoserine.
 MOD_RES 138 138 Phosphothreonine.  

Keyword

 Centromere; Chromosome; Complete proteome; Direct protein sequencing; DNA-binding; Mitochondrion; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 351 AA 

Protein Sequence

Gene Ontology

 GO:0000776; C:kinetochore; IPI:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0019237; F:centromeric DNA binding; IDA:SGD.
 GO:0001102; F:RNA polymerase II activating transcription factor binding; IMP:SGD.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:SGD.
 GO:0001078; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; IDA:SGD.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:SGD.
 GO:0001103; F:RNA polymerase II repressing transcription factor binding; IDA:SGD.
 GO:0001076; F:RNA polymerase II transcription factor binding transcription factor activity; IMP:SGD.
 GO:0006338; P:chromatin remodeling; IDA:SGD.
 GO:0007059; P:chromosome segregation; IMP:SGD.
 GO:0061427; P:negative regulation of ceramide biosynthetic process by negative regulation of transcription from RNA Polymerase II promoter; IMP:SGD.
 GO:1900375; P:positive regulation of inositol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:1900478; P:positive regulation of sulfate assimilation by positive regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0061432; P:regulation of transcription from RNA polymerase II promoter in response to methionine; IMP:SGD. 

Interpro

 IPR011598; bHLH_dom. 

Pfam

 PF00010; HLH 

SMART

 SM00353; HLH 

PROSITE

 PS50888; BHLH 

PRINTS