CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007887
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Structural maintenance of chromosomes protein 3 
Protein Synonyms/Alias
 DA-box protein SMC3 
Gene Name
 SMC3 
Gene Synonyms/Alias
 YJL074C; J1049 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
112IRRTVGLKKDDYQLNacetylation[1, 2, 3, 4, 5]
113RRTVGLKKDDYQLNDacetylation[1, 2, 3, 4, 5]
Reference
 [1] Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast.
 Zhang J, Shi X, Li Y, Kim BJ, Jia J, Huang Z, Yang T, Fu X, Jung SY, Wang Y, Zhang P, Kim ST, Pan X, Qin J.
 Mol Cell. 2008 Jul 11;31(1):143-51. [PMID: 18614053]
 [2] Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion.
 Rolef Ben-Shahar T, Heeger S, Lehane C, East P, Flynn H, Skehel M, Uhlmann F.
 Science. 2008 Jul 25;321(5888):563-6. [PMID: 18653893]
 [3] Building sister chromatid cohesion: smc3 acetylation counteracts an antiestablishment activity.
 Rowland BD, Roig MB, Nishino T, Kurze A, Uluocak P, Mishra A, Beckouët F, Underwood P, Metson J, Imre R, Mechtler K, Katis VL, Nasmyth K.
 Mol Cell. 2009 Mar 27;33(6):763-74. [PMID: 19328069]
 [4] Hos1 deacetylates Smc3 to close the cohesin acetylation cycle.
 Borges V, Lehane C, Lopez-Serra L, Flynn H, Skehel M, Rolef Ben-Shahar T, Uhlmann F.
 Mol Cell. 2010 Sep 10;39(5):677-88. [PMID: 20832720]
 [5] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. 
Sequence Annotation
 NP_BIND 32 39 ATP (Potential).
 REGION 483 684 Flexible hinge.
 MOD_RES 112 112 N6-acetyllysine.
 MOD_RES 113 113 N6-acetyllysine.  
Keyword
 Acetylation; ATP-binding; Cell cycle; Cell division; Chromosome; Coiled coil; Complete proteome; Mitosis; Nucleotide-binding; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1230 AA 
Protein Sequence
MYIKRVIIKG FKTYRNETII DNFSPHQNVI IGSNGSGKSN FFAAIRFVLS DDYSNLKREE 60
RQGLIHQGSG GSVMSASVEI VFHDPDHSMI LPSGVLSRGD DEVTIRRTVG LKKDDYQLND 120
RNVTKGDIVR MLETAGFSMN NPYNIVPQGK IVALTNAKDK ERLQLLEDVV GAKSFEVKLK 180
ASLKKMEETE QKKIQINKEM GELNSKLSEM EQERKELEKY NELERNRKIY QFTLYDRELN 240
EVINQMERLD GDYNNTVYSS EQYIQELDKR EDMIDQVSKK LSSIEASLKI KNATDLQQAK 300
LRESEISQKL TNVNVKIKDV QQQIESNEEQ RNLDSATLKE IKSIIEQRKQ KLSKILPRYQ 360
ELTKEEAMYK LQLASLQQKQ RDLILKKGEY ARFKSKDERD TWIHSEIEEL KSSIQNLNEL 420
ESQLQMDRTS LRKQYSAIDE EIEELIDSIN GPDTKGQLED FDSELIHLKQ KLSESLDTRK 480
ELWRKEQKLQ TVLETLLSDV NQNQRNVNET MSRSLANGII NVKEITEKLK ISPESVFGTL 540
GELIKVNDKY KTCAEVIGGN SLFHIVVDTE ETATLIMNEL YRMKGGRVTF IPLNRLSLDS 600
DVKFPSNTTT QIQFTPLIKK IKYEPRFEKA VKHVFGKTIV VKDLGQGLKL AKKHKLNAIT 660
LDGDRADKRG VLTGGYLDQH KRTRLESLKN LNESRSQHKK ILEELDFVRN ELNDIDTKID 720
QVNGNIRKVS NDRESVLTNI EVYRTSLNTK KNEKLILEES LNAIILKLEK LNTNRTFAQE 780
KLNTFENDLL QEFDSELSKE EKERLESLTK EISAAHNKLN ITSDALEGIT TTIDSLNAEL 840
ESKLIPQEND LESKMSEVGD AFIFGLQDEL KELQLEKESV EKQHENAVLE LGTVQREIES 900
LIAEETNNKK LLEKANNQQR LLLKKLDNFQ KSVEKTMIKK TTLVTRREEL QQRIREIGLL 960
PEDALVNDFS DITSDQLLQR LNDMNTEISG LKNVNKRAFE NFKKFNERRK DLAERASELD 1020
ESKDSIQDLI VKLKQQKVNA VDSTFQKVSE NFEAVFERLV PRGTAKLIIH RKNDNANDHD 1080
ESIDVDMDAE SNESQNGKDS EIMYTGVSIS VSFNSKQNEQ LHVEQLSGGQ KTVCAIALIL 1140
AIQMVDPASF YLFDEIDAAL DKQYRTAVAT LLKELSKNAQ FICTTFRTDM LQVADKFFRV 1200
KYENKISTVI EVNREEAIGF IRGSNKFAEV 1230 
Gene Ontology
 GO:0034990; C:nuclear mitotic cohesin complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; ISS:SGD.
 GO:0030437; P:ascospore formation; IMP:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051177; P:meiotic sister chromatid cohesion; IMP:SGD.
 GO:0007064; P:mitotic sister chromatid cohesion; IGI:SGD.
 GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
 GO:0007130; P:synaptonemal complex assembly; IMP:SGD. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR003395; RecF/RecN/SMC.
 IPR010935; SMC_hinge. 
Pfam
 PF06470; SMC_hinge
 PF02463; SMC_N 
SMART
 SM00968; SMC_hinge 
PROSITE
  
PRINTS