CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042911
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Tyrosine--tRNA ligase, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 YARS2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
17QGLLAAQKARGLFKDubiquitination[1]
23QKARGLFKDFFPETGacetylation[2]
32FFPETGTKIELPELFubiquitination[1]
255ITSTTGAKLGKSAGNubiquitination[1]
258TTGAKLGKSAGNAVWubiquitination[3, 4]
270AVWLNRDKTSPFELYubiquitination[1, 3, 5]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 291 AA 
Protein Sequence
GLRKAHSGAQ GLLAAQKARG LFKDFFPETG TKIELPELFD RGTASFPQTI YCGFDPTADS 60
LHVGHLLALL GLFHLQRAGH NVIALVGGAT ARLGDPSGRT KEREALETER VRANARALRL 120
GLEALAANHQ QLFTDGRSWG SFTVLDNSAW YQKQHLVDFL AAVGGHFRMG TLLSRQSVQL 180
RLKSPEGMSL AEFFYQVLQA YDFYYLFQRY GCRVQLGGSD QLGNIMSGYE FINKLTGEDV 240
FGITVPLITS TTGAKLGKSA GNAVWLNRDK TSPFELYQFF VRQPDDSVER D 291 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004831; F:tyrosine-tRNA ligase activity; IEA:InterPro.
 GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002307; Tyr-tRNA-ligase.
 IPR024088; Tyr-tRNA-ligase_bac-type. 
Pfam
 PF00579; tRNA-synt_1b 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR01040; TRNASYNTHTYR.