| Tag | Content |
|---|
| CPLM ID | CPLM-021088 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Rap guanine nucleotide exchange factor 4 |
Protein Synonyms/Alias | Exchange factor directly activated by cAMP 2; Exchange protein directly activated by cAMP 2; EPAC 2; cAMP-dependent Rap1 guanine-nucleotide exchange factor; cAMP-regulated guanine nucleotide exchange factor II; cAMP-GEFII |
Gene Name | Rapgef4 |
Gene Synonyms/Alias | Cgef2; Epac2 |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 184 | KENVPSEKILRAGKI | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA- independent exocytosis through interaction with RIMS2. |
Sequence Annotation | DOMAIN 216 291 DEP.
DOMAIN 496 634 N-terminal Ras-GEF.
DOMAIN 772 1009 Ras-GEF.
NP_BIND 43 166 cAMP 1.
NP_BIND 356 476 cAMP 2.
MOD_RES 681 681 Phosphotyrosine. |
Keyword | 3D-structure; Alternative promoter usage; Alternative splicing; cAMP; cAMP-binding; Complete proteome; Cytoplasm; Exocytosis; Guanine-nucleotide releasing factor; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1011 AA |
Protein Sequence | MVAAHAAHSQ SSAEWIACLD KRPLERSSED VDIIFTRLKG VKAFEKFHPN LLRQICLCGY 60
YENLEKGITL FRQGDIGTNW YAVLAGSLDV KVSETSSHQD AVTICTLGIG TAFGESILDN 120
TPRHATIVTR ESSELLRIEQ EDFKALWEKY RQYMAGLLAP PYGVMETGSN NDRIPDKENV 180
PSEKILRAGK ILRIAILSRA PHMIRDRKYH LKTYRQCCVG TELVDWMIQQ TSCVHSRTQA 240
VGMWQVLLED GVLNHVDQER HFQDKYLFYR FLDDEREDAP LPTEEEKKEC DEELQDTMLL 300
LSQMGPDAHM RMILRKPPGQ RTVDDLEIIY DELLHIKALS HLSTTVKREL AGVLIFESHA 360
KGGTVLFNQG EEGTSWYIIL KGSVNVVIYG KGVVCTLHEG DDFGKLALVN DAPRAASIVL 420
REDNCHFLRV DKEDFNRILR DVEANTVRLK EHDQDVLVLE KVPAGNRAAN QGNSQPQQKY 480
TVMSGTPEKI LEHFLETIRL EPSLNEATDS VLNDFVMMHC VFMPNTQLCP ALVAHYHAQP 540
SQGTEQERMD YALNNKRRVI RLVLQWAAMY GDLLQEDDVA MAFLEEFYVS VSDDARMMAA 600
FKEQLPELEK IVKQISEDAK APQKKHKVLL QQFNTGDERA QKRQPIRGSD EVLFKVYCID 660
HTYTTIRVPV AASVKEVISA VADKLGSGEG LIIVKMNSGG EKVVLKSNDV SVFTTLTING 720
RLFACPREQF DSLTPLPEQE GPTTGTVGTF ELMSSKDLAY QMTTYDWELF NCVHELELIY 780
HTFGRHNFKK TTANLDLFLR RFNEIQFWVV TEVCLCSQLS KRVQLLKKFI KIAAHCKEYK 840
NLNSFFAIVM GLSNVAVSRL ALTWEKLPSK FKKFYAEFES LMDPSRNHRA YRLTAAKLEP 900
PLIPFMPLLI KDMTFTHEGN KTFIDNLVNF EKMRMIANTA RTVRYYRSQP FNPDAAQANK 960
NHQDVRSYVR QLNVIDNQRT LSQMSHRLEP RRP 993 |
Gene Ontology | GO:0005952; C:cAMP-dependent protein kinase complex; IEA:InterPro. GO:0005829; C:cytosol; IDA:MGI. GO:0016020; C:membrane; IEA:UniProtKB-SubCell. GO:0030552; F:cAMP binding; IDA:UniProtKB. GO:0008603; F:cAMP-dependent protein kinase regulator activity; IEA:InterPro. GO:0005088; F:Ras guanyl-nucleotide exchange factor activity; IDA:UniProtKB. GO:0017156; P:calcium ion-dependent exocytosis; IDA:MGI. GO:0019933; P:cAMP-mediated signaling; IDA:MGI. GO:0030073; P:insulin secretion; IDA:MGI. GO:0017157; P:regulation of exocytosis; IDA:MGI. GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro. GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |