UniProt Accession

 PIMT_RAT; P22062; Q5M7V6 

Genbank Protein ID

 M26686; D11475; BC088417 

Genbank Nucleotide ID

 AAA60742.1; BAA02034.1; AAH88417.1 

Protein Name

 Protein-L-isoaspartate(D-aspartate) O-methyltransferase 

Protein Synonyms/Alias

 PIMT; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase 

Gene Name

 Pcmt1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
113	VIGIDHIKELVDDSI	acetylation	[1]
124	DDSITNVKKDDPMLL	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity). 

Sequence Annotation

 ACT_SITE 60 60 By similarity.
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 227 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004719; F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity; IDA:RGD.
 GO:0046498; P:S-adenosylhomocysteine metabolic process; IDA:RGD.
 GO:0046500; P:S-adenosylmethionine metabolic process; IDA:RGD. 

Interpro

 IPR000682; PCMT. 

Pfam

 PF01135; PCMT 

SMART

  

PROSITE

 PS01279; PCMT 

PRINTS