CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019764
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein Gfi-1 
Protein Synonyms/Alias
 Growth factor independent protein 1; Zinc finger protein 163 
Gene Name
 GFI1 
Gene Synonyms/Alias
 ZNF163 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
171PAALYGPKRAAGGAGubiquitination[1]
315QERSFDCKICGKSFKubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2- GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin- mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1- dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells. 
Sequence Annotation
 ZN_FING 255 278 C2H2-type 1.
 ZN_FING 284 306 C2H2-type 2.
 ZN_FING 312 334 C2H2-type 3.
 ZN_FING 340 362 C2H2-type 4.
 ZN_FING 368 390 C2H2-type 5.
 ZN_FING 396 419 C2H2-type 6.
 REGION 1 20 SNAG domain (By similarity).
 REGION 140 257 Required for interaction with RELA.  
Keyword
 Complete proteome; Disease mutation; DNA-binding; Metal-binding; Nucleus; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 422 AA 
Protein Sequence
MPRSFLVKSK KAHSYHQPRS PGPDYSLRLE NVPAPSRADS TSNAGGAKAE PRDRLSPESQ 60
LTEAPDRASA SPDSCEGSVC ERSSEFEDFW RPPSPSASPA SEKSMCPSLD EAQPFPLPFK 120
PYSWSGLAGS DLRHLVQSYR PCGALERGAG LGLFCEPAPE PGHPAALYGP KRAAGGAGAG 180
APGSCSAGAG ATAGPGLGLY GDFGSAAAGL YERPTAAAGL LYPERGHGLH ADKGAGVKVE 240
SELLCTRLLL GGGSYKCIKC SKVFSTPHGL EVHVRRSHSG TRPFACEMCG KTFGHAVSLE 300
QHKAVHSQER SFDCKICGKS FKRSSTLSTH LLIHSDTRPY PCQYCGKRFH QKSDMKKHTF 360
IHTGEKPHKC QVCGKAFSQS SNLITHSRKH TGFKPFGCDL CGKGFQRKVD LRRHRETQHG 420
LK 422 
Gene Ontology
 GO:0016604; C:nuclear body; IDA:UniProtKB.
 GO:0016363; C:nuclear matrix; IDA:UniProtKB.
 GO:0017053; C:transcriptional repressor complex; IDA:UniProtKB.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:BHF-UCL.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0042491; P:auditory receptor cell differentiation; IEA:Compara.
 GO:0045165; P:cell fate commitment; IEA:Compara.
 GO:0071222; P:cellular response to lipopolysaccharide; IEP:UniProtKB.
 GO:0042472; P:inner ear morphogenesis; IEA:Compara.
 GO:0007638; P:mechanosensory behavior; IEA:Compara.
 GO:0010956; P:negative regulation of calcidiol 1-monooxygenase activity; IDA:BHF-UCL.
 GO:0009996; P:negative regulation of cell fate specification; IEA:Compara.
 GO:0010977; P:negative regulation of neuron projection development; IDA:UniProtKB.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0010957; P:negative regulation of vitamin D biosynthetic process; IC:BHF-UCL.
 GO:0042660; P:positive regulation of cell fate specification; IEA:Compara.
 GO:0070105; P:positive regulation of interleukin-6-mediated signaling pathway; IDA:UniProtKB.
 GO:0034121; P:regulation of toll-like receptor signaling pathway; IDA:UniProtKB.
 GO:0000083; P:regulation of transcription involved in G1/S phase of mitotic cell cycle; TAS:ProtInc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0016032; P:viral reproduction; TAS:ProtInc. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd.
 IPR001878; Znf_CCHC. 
Pfam
 PF00096; zf-C2H2 
SMART
 SM00355; ZnF_C2H2
 SM00343; ZnF_C2HC 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS