CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021404
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rab GTPase-binding effector protein 2 
Protein Synonyms/Alias
 Rabaptin-5beta 
Gene Name
 RABEP2 
Gene Synonyms/Alias
 RABPT5B 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
105EAQITALKQERQQQQubiquitination[1, 2, 3, 4, 5]
126ERELGRLKQLLSRAYubiquitination[4]
497RVQQEQSKAQLPDLLubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Plays a role in membrane trafficking and in homotypic early endosome fusion. 
Sequence Annotation
  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Endocytosis; Endosome; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 569 AA 
Protein Sequence
MAAAAPVAAD DDERRRRPGA ALEDSRSQEG ANGEAESGEL SRLRAELAGA LAEMETMKAV 60
AEVSESTKAE AVAAVQRQCQ EEVASLQAIL KDSISSYEAQ ITALKQERQQ QQQDCEEKER 120
ELGRLKQLLS RAYPLDSLEK QMEKAHEDSE KLREIVLPME KEIEELKAKL LRAEELIQEI 180
QRRPRHAPSL HGSTELLPLS RDPSPPLEPL EELSGDGGPA AEAFAHNCDD SASISSFSLG 240
GGVGSSSSLP QSRQGLSPEQ EETASLVSTG TLVPEGIYLP PPGYQLVPDT QWEQLQTEGR 300
QLQKDLESVS RERDELQEGL RRSNEDCAKQ MQVLLAQVQN SEQLLRTLQG TVSQAQERVQ 360
LQMAELVTTH KCLHHEVKRL NEENQGLRAE QLPSSAPQGS QQEQGEEESL PSSVPELQQL 420
LCCTRQEARA RLQAQEHGAE RLRIEIVTLR EALEEETVAR ASLEGQLRVQ REETEVLEAS 480
LCSLRTEMER VQQEQSKAQL PDLLSEQRAK VLRLQAELET SEQVQRDFVR LSQALQVRLE 540
RIRQAETLEQ VRSIMDEAPL TDVRDIKDT 569 
Gene Ontology
 GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
 GO:0005096; F:GTPase activator activity; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR003914; Rabaptin.
 IPR018514; Rabaptin_coiled-coil.
 IPR015390; Rabaptin_Rab5-bd_dom. 
Pfam
 PF09311; Rab5-bind
 PF03528; Rabaptin 
SMART
  
PROSITE
  
PRINTS
 PR01432; RABAPTIN.