CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005587
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aryl hydrocarbon receptor nuclear translocator 
Protein Synonyms/Alias
 ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta 
Gene Name
 ARNT 
Gene Synonyms/Alias
 BHLHE2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
240TGRILDLKTGTVKKEubiquitination[1]
245DLKTGTVKKEGQQSSsumoylation[2]
417DSFQQVVKLKGQVLSubiquitination[1]
419FQQVVKLKGQVLSVMubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] The aryl hydrocarbon receptor nuclear transporter is modulated by the SUMO-1 conjugation system.
 Tojo M, Matsuzaki K, Minami T, Honda Y, Yasuda H, Chiba T, Saya H, Fujii-Kuriyama Y, Nakao M.
 J Biol Chem. 2002 Nov 29;277(48):46576-85. [PMID: 12354770] 
Functional Description
 Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia. 
Sequence Annotation
 DOMAIN 89 142 bHLH.
 DOMAIN 161 235 PAS 1.
 DOMAIN 349 419 PAS 2.
 DOMAIN 424 467 PAC.
 MOD_RES 77 77 Phosphoserine.  
Keyword
 3D-structure; Activator; Alternative splicing; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 789 AA 
Protein Sequence
MAATTANPEM TSDVPSLGPA IASGNSGPGI QGGGAIVQRA IKRRPGLDFD DDGEGNSKFL 60
RCDDDQMSND KERFARSDDE QSSADKERLA RENHSEIERR RRNKMTAYIT ELSDMVPTCS 120
ALARKPDKLT ILRMAVSHMK SLRGTGNTST DGSYKPSFLT DQELKHLILE AADGFLFIVS 180
CETGRVVYVS DSVTPVLNQP QSEWFGSTLY DQVHPDDVDK LREQLSTSEN ALTGRILDLK 240
TGTVKKEGQQ SSMRMCMGSR RSFICRMRCG SSSVDPVSVN RLSFVRNRCR NGLGSVKDGE 300
PHFVVVHCTG YIKAWPPAGV SLPDDDPEAG QGSKFCLVAI GRLQVTSSPN CTDMSNVCQP 360
TEFISRHNIE GIFTFVDHRC VATVGYQPQE LLGKNIVEFC HPEDQQLLRD SFQQVVKLKG 420
QVLSVMFRFR SKNQEWLWMR TSSFTFQNPY SDEIEYIICT NTNVKNSSQE PRPTLSNTIQ 480
RPQLGPTANL PLEMGSGQLA PRQQQQQTEL DMVPGRDGLA SYNHSQVVQP VTTTGPEHSK 540
PLEKSDGLFA QDRDPRFSEI YHNINADQSK GISSSTVPAT QQLFSQGNTF PPTPRPAENF 600
RNSGLAPPVT IVQPSASAGQ MLAQISRHSN PTQGATPTWT PTTRSGFSAQ QVATQATAKT 660
RTSQFGVGSF QTPSSFSSMS LPGAPTASPG AAAYPSLTNR GSNFAPETGQ TAGQFQTRTA 720
EGVGVWPQWQ GQQPHHRSSS SEQHVQQPPA QQPGQPEVFQ EMLSMLGDQS NSYNNEEFPD 780
LTMFPPFSE 789 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005667; C:transcription factor complex; IEA:InterPro.
 GO:0004874; F:aryl hydrocarbon receptor activity; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0030154; P:cell differentiation; IEA:Compara.
 GO:0001892; P:embryonic placenta development; IEA:Compara.
 GO:0042789; P:mRNA transcription from RNA polymerase II promoter; IC:BHF-UCL.
 GO:0001938; P:positive regulation of endothelial cell proliferation; IC:BHF-UCL.
 GO:0045648; P:positive regulation of erythrocyte differentiation; IC:BHF-UCL.
 GO:0045821; P:positive regulation of glycolysis; IC:BHF-UCL.
 GO:0046886; P:positive regulation of hormone biosynthetic process; IDA:BHF-UCL.
 GO:0033235; P:positive regulation of protein sumoylation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:BHF-UCL.
 GO:0030949; P:positive regulation of vascular endothelial growth factor receptor signaling pathway; IC:BHF-UCL.
 GO:0010575; P:positive regulation vascular endothelial growth factor production; IDA:BHF-UCL.
 GO:0061418; P:regulation of transcription from RNA polymerase II promoter in response to hypoxia; TAS:Reactome.
 GO:0043619; P:regulation of transcription from RNA polymerase II promoter in response to oxidative stress; IDA:BHF-UCL. 
Interpro
 IPR011598; bHLH_dom.
 IPR001067; Nuc_translocat.
 IPR001610; PAC.
 IPR000014; PAS.
 IPR013767; PAS_fold.
 IPR013655; PAS_fold_3. 
Pfam
 PF00010; HLH
 PF00989; PAS
 PF08447; PAS_3 
SMART
 SM00353; HLH
 SM00086; PAC
 SM00091; PAS 
PROSITE
 PS50888; BHLH
 PS50113; PAC
 PS50112; PAS 
PRINTS
 PR00785; NCTRNSLOCATR.