| Tag | Content |
|---|
| CPLM ID | CPLM-007761 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ubiquitin carboxyl-terminal hydrolase 5 |
Protein Synonyms/Alias | Deubiquitinating enzyme 5; Isopeptidase T; Ubiquitin thioesterase 5; Ubiquitin-specific-processing protease 5 |
Gene Name | USP5 |
Gene Synonyms/Alias | ISOT |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 163 | SADSASRKQEVQAWD | ubiquitination | [1] | | 184 | SKHAFSLKQLDNPAR | ubiquitination | [1] | | 247 | TGYPLAVKLGTITPD | ubiquitination | [1] | | 318 | QESGVPLKPLFGPGY | ubiquitination | [1] | | 353 | SIPDFQRKYVDKLEK | ubiquitination | [1] | | 357 | FQRKYVDKLEKIFQN | ubiquitination | [1] | | 360 | KYVDKLEKIFQNAPT | ubiquitination | [1] | | 379 | DFSTQVAKLGHGLLS | ubiquitination | [1] | | 391 | LLSGEYSKPVPESGD | ubiquitination | [1] | | 418 | GIAPRMFKALIGKGH | ubiquitination | [1] | | 423 | MFKALIGKGHPEFST | ubiquitination | [1] | | 476 | IKCLATEKVKYTQRV | ubiquitination | [1] | | 499 | PMDAALNKEELLEYE | ubiquitination | [1] | | 558 | QAKSVAVKTTRFASF | ubiquitination | [1] | | 574 | DYLVIQIKKFTFGLD | ubiquitination | [1] | | 575 | YLVIQIKKFTFGLDW | ubiquitination | [1] | | 585 | FGLDWVPKKLDVSIE | ubiquitination | [1] | | 586 | GLDWVPKKLDVSIEM | ubiquitination | [1] | | 720 | FSRDQALKALRATNN | ubiquitination | [1] | | 770 | ESVPVGPKVRDGPGK | ubiquitination | [1] |
|
Reference | [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization. Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW. Nature. 2013 Apr 18;496(7445):372-6. [ PMID: 23503661] |
Functional Description | Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. |
Sequence Annotation | DOMAIN 654 695 UBA 1.
DOMAIN 722 762 UBA 2.
ZN_FING 197 269 UBP-type.
REGION 221 224 Substrate binding.
ACT_SITE 335 335 Nucleophile.
ACT_SITE 818 818 Proton acceptor (By similarity).
METAL 199 199 Zinc.
METAL 202 202 Zinc.
METAL 219 219 Zinc.
METAL 232 232 Zinc.
BINDING 209 209 Substrate.
BINDING 259 259 Substrate.
BINDING 261 261 Substrate; via carbonyl oxygen.
BINDING 264 264 Substrate.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 623 623 Phosphothreonine (By similarity).
MOD_RES 783 783 Phosphoserine.
DISULFID 195 816 |
Keyword | 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Disulfide bond; Hydrolase; Metal-binding; Phosphoprotein; Protease; Reference proteome; Repeat; Thiol protease; Ubl conjugation pathway; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 858 AA |
Protein Sequence | MAELSEEALL SVLPTIRVPK AGDRVHKDEC AFSFDTPESE GGLYICMNTF LGFGKQYVER 60
HFNKTGQRVY LHLRRTRRPK EEDPATGTGD PPRKKPTRLA IGVEGGFDLS EEKFELDEDV 120
KIVILPDYLE IARDGLGGLP DIVRDRVTSA VEALLSADSA SRKQEVQAWD GEVRQVSKHA 180
FSLKQLDNPA RIPPCGWKCS KCDMRENLWL NLTDGSILCG RRYFDGSGGN NHAVEHYRET 240
GYPLAVKLGT ITPDGADVYS YDEDDMVLDP SLAEHLSHFG IDMLKMQKTD KTMTELEIDM 300
NQRIGEWELI QESGVPLKPL FGPGYTGIRN LGNSCYLNSV VQVLFSIPDF QRKYVDKLEK 360
IFQNAPTDPT QDFSTQVAKL GHGLLSGEYS KPVPESGDGE RVPEQKEVQD GIAPRMFKAL 420
IGKGHPEFST NRQQDAQEFF LHLINMVERN CRSSENPNEV FRFLVEEKIK CLATEKVKYT 480
QRVDYIMQLP VPMDAALNKE ELLEYEEKKR QAEEEKMALP ELVRAQVPFS SCLEAYGAPE 540
QVDDFWSTAL QAKSVAVKTT RFASFPDYLV IQIKKFTFGL DWVPKKLDVS IEMPEELDIS 600
QLRGTGLQPG EEELPDIAPP LVTPDEPKAP MLDESVIIQL VEMGFPMDAC RKAVYYTGNS 660
GAEAAMNWVM SHMDDPDFAN PLILPGSSGP GSTSAAADPP PEDCVTTIVS MGFSRDQALK 720
ALRATNNSLE RAVDWIFSHI DDLDAEAAMD ISEGRSAADS ISESVPVGPK VRDGPGKYQL 780
FAFISHMGTS TMCGHYVCHI KKEGRWVIYN DQKVCASEKP PKDLGYIYFY QRVAS 835 |
Gene Ontology | GO:0005764; C:lysosome; TAS:ProtInc. GO:0004197; F:cysteine-type endopeptidase activity; TAS:ProtInc. GO:0008242; F:omega peptidase activity; IEA:InterPro. GO:0004221; F:ubiquitin thiolesterase activity; TAS:ProtInc. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IMP:UniProtKB. GO:0071108; P:protein K48-linked deubiquitination; IMP:UniProtKB. GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |