UniProt Accession

 CCA_BACSU; P42977 

Genbank Protein ID

 L38424; L47709; AL009126 

Genbank Nucleotide ID

 AAA92878.1; AAB38446.1; CAB14161.1 

Protein Name

 CCA-adding enzyme 

Protein Synonyms/Alias

 CCA tRNA nucleotidyltransferase; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT 

Gene Name

 cca 

Gene Synonyms/Alias

 papS; ypjI; BSU22450 

Created Date

 July 27, 2013 

Organism

 Bacillus subtilis (strain 168) 

NCBI Taxa ID

 224308 

Lysine Modification

Position	Peptide	Type	References
64	QRTVDVGKEHGTIIV	acetylation	[1]
182	ETEEAIAKEKSLLSH	acetylation	[1]

Reference

 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 

Functional Description

 Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Has no poly(A) polymerase activity. 

Sequence Annotation

 METAL 40 40 Magnesium (By similarity).
 METAL 42 42 Magnesium (By similarity).
 BINDING 27 27 ATP or CTP; via amide nitrogen (By
 BINDING 30 30 ATP or CTP (By similarity).
 BINDING 111 111 ATP or CTP (By similarity).
 BINDING 154 154 ATP or CTP (By similarity).
 BINDING 157 157 ATP or CTP (By similarity).
 BINDING 160 160 ATP or CTP (By similarity).
 BINDING 163 163 ATP or CTP (By similarity).  

Keyword

 ATP-binding; Complete proteome; Magnesium; Metal-binding; Nucleotide-binding; Nucleotidyltransferase; Reference proteome; RNA repair; RNA-binding; Transferase; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 397 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0052929; F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity; IEA:EC.
 GO:0052928; F:CTP:3'-cytidine-tRNA cytidylyltransferase activity; IEA:EC.
 GO:0052927; F:CTP:tRNA cytidylyltransferase activity; IEA:EC.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0004810; F:tRNA adenylyltransferase activity; IEA:HAMAP.
 GO:0000049; F:tRNA binding; IEA:HAMAP.
 GO:0016437; F:tRNA cytidylyltransferase activity; IEA:HAMAP.
 GO:0042245; P:RNA repair; IEA:UniProtKB-KW.
 GO:0001680; P:tRNA 3'-terminal CCA addition; IEA:HAMAP. 

Interpro

 IPR023068; CCA-adding_enz_firmicutes.
 IPR026007; PcnB/CCA-adding.
 IPR002646; PolA_pol_head_dom. 

Pfam

 PF01743; PolyA_pol 

SMART

  

PROSITE

  

PRINTS