UniProt Accession

 PABP1_RAT; Q9EPH8 

Genbank Protein ID

 AJ298278; BC083176 

Genbank Nucleotide ID

 CAC21554.1; AAH83176.1 

Protein Name

 Polyadenylate-binding protein 1 

Protein Synonyms/Alias

 PABP-1; Poly(A)-binding protein 1 

Gene Name

 Pabpc1 

Gene Synonyms/Alias

 Pabp1 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
188	AELGARAKEFTNVYI	ubiquitination	[1]
259	NGKELNGKQIYVGRA	acetylation	[2]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre- mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (By similarity). 

Sequence Annotation

 DOMAIN 11 89 RRM 1.
 DOMAIN 99 175 RRM 2.
 DOMAIN 191 268 RRM 3.
 DOMAIN 294 370 RRM 4.
 DOMAIN 542 619 PABC.
 REGION 166 289 UNR-binding (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 116 116 Phosphotyrosine (By similarity).
 MOD_RES 299 299 N6-methyllysine (By similarity).
 MOD_RES 315 315 Phosphoserine (By similarity).
 MOD_RES 455 455 Omega-N-methylated arginine; by CARM1 (By
 MOD_RES 460 460 Omega-N-methylated arginine; by CARM1 (By
 MOD_RES 493 493 Dimethylated arginine; alternate (By
 MOD_RES 493 493 Omega-N-methylarginine; alternate (By
 MOD_RES 512 512 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Methylation; mRNA processing; mRNA splicing; Nonsense-mediated mRNA decay; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 636 AA 

Protein Sequence

Gene Ontology

 GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0008143; F:poly(A) RNA binding; ISS:UniProtKB.
 GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:2000623; P:negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISS:UniProtKB.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
 GO:1900153; P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; ISS:UniProtKB.
 GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; ISS:UniProtKB.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR006515; PABP_1234.
 IPR002004; PABP_HYD.
 IPR000504; RRM_dom. 

Pfam

 PF00658; PABP
 PF00076; RRM_1 

SMART

 SM00517; PolyA
 SM00360; RRM 

PROSITE

 PS51309; PABC
 PS50102; RRM 

PRINTS