CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013848
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NAD-dependent protein deacetylase sirtuin-2 
Protein Synonyms/Alias
 Regulatory protein SIR2 homolog 2; SIR2-like protein 2 
Gene Name
 Sirt2 
Gene Synonyms/Alias
 Sir2l2 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
18TLGLGSQKERLLDELacetylation[1]
18TLGLGSQKERLLDELubiquitination[2]
89FEISYFKKHPEPFFAubiquitination[2]
121FIRLLKEKGLLLRCYacetylation[1]
173EYTMSWMKEKIFSEAacetylation[1]
175TMSWMKEKIFSEATPacetylation[1]
183IFSEATPKCEKCQNVacetylation[1]
250TPRLLINKEKTGQTDacetylation[1]
250TPRLLINKEKTGQTDubiquitination[2]
301LADLLGWKELEDLVRacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113
Functional Description
 NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA (By similarity). 
Sequence Annotation
 DOMAIN 28 303 Deacetylase sirtuin-type.
 NP_BIND 47 67 NAD (By similarity).
 NP_BIND 130 133 NAD (By similarity).
 NP_BIND 224 226 NAD (By similarity).
 NP_BIND 249 251 NAD (By similarity).
 ACT_SITE 150 150 Proton acceptor (By similarity).
 METAL 158 158 Zinc (By similarity).
 METAL 163 163 Zinc (By similarity).
 METAL 184 184 Zinc (By similarity).
 METAL 187 187 Zinc (By similarity).
 BINDING 287 287 NAD; via amide nitrogen (By similarity).  
Keyword
 Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Hydrolase; Metal-binding; Microtubule; Mitosis; NAD; Phosphoprotein; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 350 AA 
Protein Sequence
MDFLRNLFTQ TLGLGSQKER LLDELTLEGV TRYMQSERCR RVICLVGAGI STSAGIPDFR 60
SPSTGLYANL EKYHLPYPEA IFEISYFKKH PEPFFALAKE LYPGQFKPTI CHYFIRLLKE 120
KGLLLRCYTQ NIDTLERVAG LEPQDLVEAH GTFYTSHCVN TSCGKEYTMS WMKEKIFSEA 180
TPKCEKCQNV VKPDIVFFGE NLPPRFFSCM QSDFSKVDLL IIMGTSLQVQ PFASLISKAP 240
LATPRLLINK EKTGQTDPFL GMMMGLGGGM DFDSKKAYRD VAWLGDCDQG CLALADLLGW 300
KELEDLVRRE HANIDAQSGS QASNPSATVS PRKSPPPAKE AARTKEKEEH 350 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0070403; F:NAD+ binding; IEA:InterPro.
 GO:0017136; F:NAD-dependent histone deacetylase activity; IEA:Compara.
 GO:0042903; F:tubulin deacetylase activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:Compara.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0045843; P:negative regulation of striated muscle tissue development; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB. 
Interpro
 IPR017328; NAD-dep_deAcase_SIR2_class_I.
 IPR003000; Sirtuin.
 IPR026590; Ssirtuin_cat_dom. 
Pfam
 PF02146; SIR2 
SMART
  
PROSITE
 PS50305; SIRTUIN 
PRINTS