UniProt Accession

 ADT2_MOUSE; P51881; Q61311 

Genbank Protein ID

 U27316; U10404; X70847; AF240003; BC004570; BC086756 

Genbank Nucleotide ID

 AAC52838.1; AAA19009.1; CAA50196.1; AAF64471.1; AAH04570.1; AAH86756.1 

Protein Name

 ADP/ATP translocase 2 

Protein Synonyms/Alias

 ADP,ATP carrier protein 2; Adenine nucleotide translocator 2; ANT 2; Solute carrier family 25 member 5; ADP/ATP translocase 2, N-terminally processed 

Gene Name

 Slc25a5 

Gene Synonyms/Alias

 Ant2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
10	DAAVSFAKDFLAGGV	acetylation	[1, 2, 3]
10	DAAVSFAKDFLAGGV	ubiquitination	[4]
23	GVAAAISKTAVAPIE	acetylation	[2, 3, 5, 6, 7, 8, 9, 10]
23	GVAAAISKTAVAPIE	succinylation	[9]
23	GVAAAISKTAVAPIE	ubiquitination	[4]
33	VAPIERVKLLLQVQH	acetylation	[7]
43	LQVQHASKQITADKQ	acetylation	[3, 6, 7, 9]
43	LQVQHASKQITADKQ	succinylation	[9]
43	LQVQHASKQITADKQ	ubiquitination	[4]
49	SKQITADKQYKGIID	acetylation	[3, 10]
52	ITADKQYKGIIDCVV	acetylation	[3]
63	DCVVRIPKEQGVLSF	acetylation	[2, 3, 6, 9]
63	DCVVRIPKEQGVLSF	ubiquitination	[4]
92	QALNFAFKDKYKQIF	acetylation	[7]
94	LNFAFKDKYKQIFLG	acetylation	[6, 7]
96	FAFKDKYKQIFLGGV	acetylation	[1, 3, 6, 7, 8, 9, 10]
96	FAFKDKYKQIFLGGV	phosphoglycerylation	[11]
96	FAFKDKYKQIFLGGV	ubiquitination	[4]
105	IFLGGVDKRTQFWRY	acetylation	[2, 3, 6, 7, 9, 10]
105	IFLGGVDKRTQFWRY	phosphoglycerylation	[11]
105	IFLGGVDKRTQFWRY	succinylation	[9]
147	RLAADVGKAGAEREF	acetylation	[2, 3, 7, 9]
147	RLAADVGKAGAEREF	succinylation	[9]
147	RLAADVGKAGAEREF	ubiquitination	[4]
155	AGAEREFKGLGDCLV	acetylation	[2, 3, 5, 6, 7, 8, 9, 10, 12]
155	AGAEREFKGLGDCLV	succinylation	[9]
155	AGAEREFKGLGDCLV	ubiquitination	[4]
163	GLGDCLVKIYKSDGI	acetylation	[2, 3, 5, 6, 7, 8, 9, 10, 12]
163	GLGDCLVKIYKSDGI	succinylation	[9]
163	GLGDCLVKIYKSDGI	ubiquitination	[4]
166	DCLVKIYKSDGIKGL	acetylation	[3, 5, 7, 9, 10]
166	DCLVKIYKSDGIKGL	succinylation	[9]
199	FGIYDTAKGMLPDPK	acetylation	[2, 5, 6, 8]
245	MMMQSGRKGTDIMYT	acetylation	[9]
245	MMMQSGRKGTDIMYT	succinylation	[9]
268	IARDEGSKAFFKGAW	acetylation	[2, 3, 7, 9, 10]
268	IARDEGSKAFFKGAW	succinylation	[9]
268	IARDEGSKAFFKGAW	ubiquitination	[4]
272	EGSKAFFKGAWSNVL	acetylation	[7]

Reference

 [1] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [6] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [7] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [10] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [11] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
 [12] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599] 

Functional Description

 Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (By similarity). 

Sequence Annotation

 REPEAT 6 98 Solcar 1.
 REPEAT 111 201 Solcar 2.
 REPEAT 212 297 Solcar 3.
 MOTIF 235 240 Substrate recognition (By similarity).
 BINDING 80 80 Nucleotide (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 2 2 N-acetylthreonine; in ADP/ATP translocase
 MOD_RES 23 23 N6-acetyllysine; alternate (Probable).
 MOD_RES 23 23 N6-malonyllysine; alternate (By
 MOD_RES 42 42 Phosphoserine.
 MOD_RES 52 52 N6,N6-dimethyllysine; alternate (By
 MOD_RES 52 52 N6-methyllysine; alternate (By
 MOD_RES 92 92 N6-acetyllysine; alternate (Probable).
 MOD_RES 92 92 N6-malonyllysine; alternate (By
 MOD_RES 96 96 N6-malonyllysine (By similarity).
 MOD_RES 105 105 N6-acetyllysine (By similarity).
 MOD_RES 147 147 N6-malonyllysine (By similarity).
 MOD_RES 155 155 N6-acetyllysine.
 MOD_RES 163 163 N6-acetyllysine.
 MOD_RES 166 166 N6-acetyllysine.
 MOD_RES 191 191 Phosphotyrosine.
 MOD_RES 195 195 Phosphotyrosine.
 MOD_RES 199 199 N6-acetyllysine.  

Keyword

 Acetylation; Chromosome partition; Complete proteome; Direct protein sequencing; Membrane; Methylation; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 298 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0042645; C:mitochondrial nucleoid; IEA:Compara.
 GO:0071817; C:MMXD complex; ISS:UniProtKB.
 GO:0005215; F:transporter activity; IEA:InterPro.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:1901029; P:negative regulation of mitochondrial outer membrane permeabilization; ISS:UniProtKB.
 GO:0008284; P:positive regulation of cell proliferation; ISS:UniProtKB.
 GO:0055085; P:transmembrane transport; IEA:InterPro. 

Interpro

 IPR002113; Aden_trnslctor.
 IPR002067; Mit_carrier.
 IPR018108; Mitochondrial_sb/sol_carrier.
 IPR023395; Mt_carrier_dom. 

Pfam

 PF00153; Mito_carr 

SMART

  

PROSITE

 PS50920; SOLCAR 

PRINTS

 PR00927; ADPTRNSLCASE.
 PR00926; MITOCARRIER.