CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005634
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositol 3-kinase regulatory subunit alpha 
Protein Synonyms/Alias
 PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha 
Gene Name
 PIK3R1 
Gene Synonyms/Alias
 GRB1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
438YQQDQVVKEDNIEAVubiquitination[1]
530RIMHNYDKLKSRISEacetylation[2]
550RRLEEDLKKQAAEYRubiquitination[3]
633VGSSNRNKAENLLRGubiquitination[3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. 
Sequence Annotation
 DOMAIN 3 79 SH3.
 DOMAIN 113 301 Rho-GAP.
 DOMAIN 333 428 SH2 1.
 DOMAIN 624 718 SH2 2.
 MOD_RES 467 467 Phosphotyrosine (By similarity).
 MOD_RES 580 580 Phosphotyrosine.
 MOD_RES 607 607 Phosphotyrosine (By similarity).
 MOD_RES 608 608 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Host-virus interaction; Phosphoprotein; Polymorphism; Reference proteome; Repeat; SH2 domain; SH3 domain; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 724 AA 
Protein Sequence
MSAEGYQYRA LYDYKKEREE DIDLHLGDIL TVNKGSLVAL GFSDGQEARP EEIGWLNGYN 60
ETTGERGDFP GTYVEYIGRK KISPPTPKPR PPRPLPVAPG SSKTEADVEQ QALTLPDLAE 120
QFAPPDIAPP LLIKLVEAIE KKGLECSTLY RTQSSSNLAE LRQLLDCDTP SVDLEMIDVH 180
VLADAFKRYL LDLPNPVIPA AVYSEMISLA PEVQSSEEYI QLLKKLIRSP SIPHQYWLTL 240
QYLLKHFFKL SQTSSKNLLN ARVLSEIFSP MLFRFSAASS DNTENLIKVI EILISTEWNE 300
RQPAPALPPK PPKPTTVANN GMNNNMSLQD AEWYWGDISR EEVNEKLRDT ADGTFLVRDA 360
STKMHGDYTL TLRKGGNNKL IKIFHRDGKY GFSDPLTFSS VVELINHYRN ESLAQYNPKL 420
DVKLLYPVSK YQQDQVVKED NIEAVGKKLH EYNTQFQEKS REYDRLYEEY TRTSQEIQMK 480
RTAIEAFNET IKIFEEQCQT QERYSKEYIE KFKREGNEKE IQRIMHNYDK LKSRISEIID 540
SRRRLEEDLK KQAAEYREID KRMNSIKPDL IQLRKTRDQY LMWLTQKGVR QKKLNEWLGN 600
ENTEDQYSLV EDDEDLPHHD EKTWNVGSSN RNKAENLLRG KRDGTFLVRE SSKQGCYACS 660
VVVDGEVKHC VINKTATGYG FAEPYNLYSS LKELVLHYQH TSLVQHNDSL NVTLAYPVYA 720
QQRR 724 
Gene Ontology
 GO:0005943; C:1-phosphatidylinositol-4-phosphate 3-kinase, class IA complex; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005545; F:1-phosphatidylinositol binding; NAS:UniProtKB.
 GO:0043125; F:ErbB-3 class receptor binding; IDA:UniProtKB.
 GO:0043559; F:insulin binding; IDA:UniProtKB.
 GO:0043560; F:insulin receptor substrate binding; ISS:BHF-UCL.
 GO:0043548; F:phosphatidylinositol 3-kinase binding; ISS:BHF-UCL.
 GO:0035014; F:phosphatidylinositol 3-kinase regulator activity; ISS:UniProtKB.
 GO:0005068; F:transmembrane receptor protein tyrosine kinase adaptor activity; ISS:BHF-UCL.
 GO:0030183; P:B cell differentiation; IEA:Compara.
 GO:0034644; P:cellular response to UV; IEA:Compara.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0060396; P:growth hormone receptor signaling pathway; IDA:BHF-UCL.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0008286; P:insulin receptor signaling pathway; IPI:UniProtKB.
 GO:0048009; P:insulin-like growth factor receptor signaling pathway; IPI:UniProtKB.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0001953; P:negative regulation of cell-matrix adhesion; IEA:Compara.
 GO:0045671; P:negative regulation of osteoclast differentiation; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0051531; P:NFAT protein import into nucleus; IEA:Compara.
 GO:0014065; P:phosphatidylinositol 3-kinase cascade; IDA:BHF-UCL.
 GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome.
 GO:0046854; P:phosphatidylinositol phosphorylation; ISS:UniProtKB.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0090004; P:positive regulation of establishment of protein localization to plasma membrane; ISS:BHF-UCL.
 GO:0046326; P:positive regulation of glucose import; ISS:BHF-UCL.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006468; P:protein phosphorylation; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0031295; P:T cell costimulation; TAS:Reactome.
 GO:0050852; P:T cell receptor signaling pathway; TAS:Reactome.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR001720; PI3kinase_P85.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom.
 IPR000980; SH2.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00620; RhoGAP
 PF00017; SH2
 PF07653; SH3_2 
SMART
 SM00324; RhoGAP
 SM00252; SH2
 SM00326; SH3 
PROSITE
 PS50238; RHOGAP
 PS50001; SH2
 PS50002; SH3 
PRINTS
 PR00678; PI3KINASEP85.
 PR00401; SH2DOMAIN.