CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000397
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Membrane-associated progesterone receptor component 2 
Protein Synonyms/Alias
 Progesterone membrane-binding protein; Steroid receptor protein DG6 
Gene Name
 PGRMC2 
Gene Synonyms/Alias
 DG6; PMBP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
126ILLAVNGKVFDVTKGubiquitination[1, 2, 3, 4, 5]
132GKVFDVTKGSKFYGPubiquitination[3]
135FDVTKGSKFYGPAGPubiquitination[1, 2, 4, 6]
162LATFCLDKDALRDEYubiquitination[2]
191REWEMQFKEKYDYVGubiquitination[1, 3, 4]
193WEMQFKEKYDYVGRLacetylation[5]
193WEMQFKEKYDYVGRLubiquitination[1, 3, 4, 5]
217YTDEEDTKDHNKQD*ubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 Receptor for steroids (Potential). 
Sequence Annotation
 DOMAIN 102 201 Cytochrome b5 heme-binding.
 MOD_RES 90 90 Phosphoserine.
 MOD_RES 208 208 Phosphoserine (By similarity).
 MOD_RES 210 210 Phosphotyrosine.
 MOD_RES 211 211 Phosphothreonine.  
Keyword
 Complete proteome; Lipid-binding; Membrane; Phosphoprotein; Receptor; Reference proteome; Steroid-binding; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 223 AA 
Protein Sequence
MAAGDGDVKL GTLGSGSESS NDGGSESPGD AGAAAEGGGW AAAALALLTG GGEMLLNVAL 60
VALVLLGAYR LWVRWGRRGL GAGAGAGEES PATSLPRMKK RDFSLEQLRQ YDGSRNPRIL 120
LAVNGKVFDV TKGSKFYGPA GPYGIFAGRD ASRGLATFCL DKDALRDEYD DLSDLNAVQM 180
ESVREWEMQF KEKYDYVGRL LKPGEEPSEY TDEEDTKDHN KQD 223 
Gene Ontology
 GO:0016021; C:integral to membrane; TAS:ProtInc.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005496; F:steroid binding; TAS:ProtInc.
 GO:0003707; F:steroid hormone receptor activity; TAS:ProtInc. 
Interpro
 IPR001199; Cyt_B5-like_heme/steroid-bd. 
Pfam
 PF00173; Cyt-b5 
SMART
  
PROSITE
 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2 
PRINTS