CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016463
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neutral alpha-glucosidase AB 
Protein Synonyms/Alias
 Alpha-glucosidase 2; Glucosidase II subunit alpha 
Gene Name
 Ganab 
Gene Synonyms/Alias
 G2an; Kiaa0088 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
39AVDRSNFKTCDESSFacetylation[1]
48CDESSFCKRQRSIRPacetylation[1]
220ETQEKAEKDEPGAWEacetylation[2]
462HLASKRRKLVAIVDPacetylation[1]
472AIVDPHIKVDSGYRVubiquitination[3]
908AAVVLQTKGSPESRLubiquitination[3]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. 
Sequence Annotation
 ACT_SITE 542 542 Nucleophile (By similarity).
 ACT_SITE 545 545 By similarity.
 ACT_SITE 618 618 Proton donor (By similarity).
 CARBOHYD 97 97 N-linked (GlcNAc...).  
Keyword
 Alternative splicing; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Glycosidase; Golgi apparatus; Hydrolase; Reference proteome; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 944 AA 
Protein Sequence
MAAIAAVAAR RRRSWLSLVL AYLGVCLGIT LAVDRSNFKT CDESSFCKRQ RSIRPGLSPY 60
RALLDTLQLG PDALTVHLIH EVTKVLLVLE LQGLQKNMTR IRIDELEPRR PRYRVPDVLV 120
ADPPTARLSV SGRDDNSVEL TVAEGPYKII LTAQPFRLDL LEDRSLLLSV NARGLMAFEH 180
QRAPRVPQES KDPAEGNGAQ PEATPGDGDK PEETQEKAEK DEPGAWEETF KTHSDSKPYG 240
PTSVGLDFSL PGMEHVYGIP EHADSLRLKV TEGGEPYRLY NLDVFQYELN NPMALYGSVP 300
VLLAHSFHRD LGIFWLNAAE TWVDISSNTA GKTLFGKMLD YLQGSGETPQ TDIRWMSESG 360
IIDVFLMLGP SVFDVFRQYA SLTGTQALPP LFSLGYHQSR WNYRDEADVL EVDQGFDDHN 420
MPCDVIWLDI EHADGKRYFT WDPTRFPQPL NMLEHLASKR RKLVAIVDPH IKVDSGYRVH 480
EELRNHGLYV KTRDGSDYEG WCWPGSASYP DFTNPRMRAW WSNMFSFDNY EGSAPNLYVW 540
NDMNEPSVFN GPEVTMLKDA VHYGGWEHRD IHNIYGLYVH MATADGLIQR SGGIERPFVL 600
SRAFFSGSQR FGAVWTGDNT AEWDHLKISI PMCLSLALVG LSFCGADVGG FFKNPEPELL 660
VRWYQMGAYQ PFFRAHAHLD TGRREPWLLA SQYQDAIRDA LFQRYSLLPF WYTLFYQAHK 720
EGFPVMRPLW VQYPEDMSTF SIEDQFMLGD ALLIHPVSDA GAHGVQVYLP GQEEVWYDIQ 780
SYQKHHGPQT LYLPVTLSSI PVFQRGGTIV PRWMRVRRSS DCMKDDPITL FVALSPQGTA 840
QGELFLDDGH TFNYQTRHEF LLRRFSFSGS TLVSSSADPK GHLETPIWIE RVVIMGAGKP 900
AAVVLQTKGS PESRLSFQHD PETSVLILRK PGVSVASDWS IHLR 944 
Gene Ontology
 GO:0017177; C:glucosidase II complex; IPI:MGI.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0033919; F:glucan 1,3-alpha-glucosidase activity; IEA:EC.
 GO:0015926; F:glucosidase activity; ISA:MGI.
 GO:0006491; P:N-glycan processing; TAS:MGI. 
Interpro
 IPR011013; Gal_mutarotase_SF_dom.
 IPR000322; Glyco_hydro_31.
 IPR025887; Glyco_hydro_31_N_dom.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF13802; Gal_mutarotas_2
 PF01055; Glyco_hydro_31 
SMART
  
PROSITE
 PS00129; GLYCOSYL_HYDROL_F31_1
 PS00707; GLYCOSYL_HYDROL_F31_2 
PRINTS