CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020114
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleolar protein 10 
Protein Synonyms/Alias
  
Gene Name
 NOL10 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18IYSLSCGKSLPEWLSubiquitination[1]
74ILATGTYKPRVRCYDubiquitination[1, 2, 3, 4]
262LYDLRSDKPLLVKDHubiquitination[1]
267SDKPLLVKDHQYGLPubiquitination[1]
296SADSRIVKMWNKNSGubiquitination[1]
304MWNKNSGKIFTSLEPubiquitination[1]
428YEEYRKDKIRQKIEEubiquitination[1]
464LIEEEEEKQKSTWKKubiquitination[3]
589EDQQTVLKPQFYEIKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 REPEAT 50 90 WD 1.
 REPEAT 174 213 WD 2.
 REPEAT 228 266 WD 3.
 REPEAT 270 308 WD 4.
 REPEAT 310 349 WD 5.
 MOD_RES 25 25 Phosphoserine.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 688 AA 
Protein Sequence
MQVSSLNEVK IYSLSCGKSL PEWLSDRKKR ALQKKDVDVR RRIELIQDFE MPTVCTTIKV 60
SKDGQYILAT GTYKPRVRCY DTYQLSLKFE RCLDSEVVTF EILSDDYSKI VFLHNDRYIE 120
FHSQSGFYYK TRIPKFGRDF SYHYPSCDLY FVGASSEVYR LNLEQGRYLN PLQTDAAENN 180
VCDINSVHGL FATGTIEGRV ECWDPRTRNR VGLLDCALNS VTADSEINSL PTISALKFNG 240
ALTMAVGTTT GQVLLYDLRS DKPLLVKDHQ YGLPIKSVHF QDSLDLILSA DSRIVKMWNK 300
NSGKIFTSLE PEHDLNDVCL YPNSGMLLTA NETPKMGIYY IPVLGPAPRW CSFLDNLTEE 360
LEENPESTVY DDYKFVTKKD LENLGLTHLI GSPFLRAYMH GFFMDIRLYH KVKLMVNPFA 420
YEEYRKDKIR QKIEETRAQR VQLKKLPKVN KELALKLIEE EEEKQKSTWK KKVKSLPNIL 480
TDDRFKVMFE NPDFQVDEES EEFRLLNPLV SKISEKRKKK LRLLEQQELR EKEEEEEPEG 540
KPSDAESSES SDDEKAWVEE VRKQRRLLQQ EEKVKRQERL KEDQQTVLKP QFYEIKAGEE 600
FRSFKDSATK QKLMNKTLED RLKIEAKNGT LSVSDTTVGS KQLTFTLKRS EQQKKQQEAE 660
KLHRQERKRL RRSAGHLKSR HKRGRSFH 688 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell. 
Interpro
 IPR012580; NUC153.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF08159; NUC153 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS