UniProt Accession

 MMP20_HUMAN; O60882; Q6DKT9 

Genbank Protein ID

 Y12779; AY673603; AP000851 

Genbank Nucleotide ID

 CAA73317.1; AAT70722.1 

Protein Name

 Matrix metalloproteinase-20 

Protein Synonyms/Alias

 MMP-20; Enamel metalloproteinase; Enamelysin 

Gene Name

 MMP20 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
118	FPGEPKWKKNTLTYR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. 

Sequence Annotation

 REPEAT 293 343 Hemopexin 1.
 REPEAT 344 389 Hemopexin 2.
 REPEAT 391 439 Hemopexin 3.
 REPEAT 440 483 Hemopexin 4.
 MOTIF 98 105 Cysteine switch (By similarity).
 ACT_SITE 227 227 By similarity.
 METAL 100 100 Zinc 1; in inhibited form (By
 METAL 164 164 Calcium 1.
 METAL 165 165 Calcium 1; via carbonyl oxygen.
 METAL 166 166 Calcium 1; via carbonyl oxygen.
 METAL 176 176 Zinc 2.
 METAL 178 178 Zinc 2.
 METAL 183 183 Calcium 2.
 METAL 184 184 Calcium 2; via carbonyl oxygen.
 METAL 186 186 Calcium 2; via carbonyl oxygen.
 METAL 188 188 Calcium 2; via carbonyl oxygen.
 METAL 191 191 Zinc 2.
 METAL 197 197 Calcium 1; via carbonyl oxygen.
 METAL 198 198 Calcium 1; via carbonyl oxygen.
 METAL 200 200 Calcium 1; via carbonyl oxygen.
 METAL 202 202 Calcium 1.
 METAL 204 204 Zinc 2.
 METAL 206 206 Calcium 2.
 METAL 209 209 Calcium 2.
 METAL 226 226 Zinc 1; catalytic.
 METAL 230 230 Zinc 1; catalytic.
 METAL 236 236 Zinc 1; catalytic.
 DISULFID 296 483 Potential.  

Keyword

 3D-structure; Amelogenesis imperfecta; Autocatalytic cleavage; Calcium; Complete proteome; Disulfide bond; Extracellular matrix; Hydrolase; Metal-binding; Metalloprotease; Polymorphism; Protease; Reference proteome; Repeat; Secreted; Signal; Zinc; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 483 AA 

Protein Sequence

Gene Ontology

 GO:0005615; C:extracellular space; TAS:ProtInc.
 GO:0005578; C:proteinaceous extracellular matrix; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0004222; F:metalloendopeptidase activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; TAS:ProtInc.
 GO:0097186; P:amelogenesis; IEA:Compara.
 GO:0030574; P:collagen catabolic process; TAS:Reactome.
 GO:0022617; P:extracellular matrix disassembly; TAS:Reactome.
 GO:0006508; P:proteolysis; TAS:ProtInc.
 GO:0070173; P:regulation of enamel mineralization; TAS:BHF-UCL. 

Interpro

 IPR000585; Hemopexin-like_dom.
 IPR018487; Hemopexin-like_repeat.
 IPR024079; MetalloPept_cat_dom.
 IPR001818; Pept_M10_metallopeptidase.
 IPR021190; Pept_M10A.
 IPR016293; Pept_M10A_Metazoans.
 IPR021158; Pept_M10A_Zn_BS.
 IPR006026; Peptidase_Metallo.
 IPR002477; Peptidoglycan-bd-like. 

Pfam

 PF00045; Hemopexin
 PF00413; Peptidase_M10
 PF01471; PG_binding_1 

SMART

 SM00120; HX
 SM00235; ZnMc 

PROSITE

 PS00546; CYSTEINE_SWITCH
 PS00024; HEMOPEXIN
 PS51642; HEMOPEXIN_2
 PS00142; ZINC_PROTEASE 

PRINTS

 PR00138; MATRIXIN.