UniProt Accession

 GPDA_MOUSE; P13707; Q3UVM3; Q5DTS5 

Genbank Protein ID

 J02655; M25558; M13366; AK220445; AK137134; AK004565; AK018733; BC005756; BC019391 

Genbank Nucleotide ID

 AAA37728.1; AAA37726.1; AAA37727.1; BAD90479.1; BAE23246.1; BAB23376.1; BAB31376.1; AAH05756.1; AAH19391.1 

Protein Name

 Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic 

Protein Synonyms/Alias

 GPD-C; GPDH-C 

Gene Name

 Gpd1 

Gene Synonyms/Alias

 Gdc-1; Gdc1; Kiaa4010 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
49	EEDIGGRKLTEIINT	ubiquitination	[1]
62	NTQHENVKYLPGHKL	acetylation	[2]
62	NTQHENVKYLPGHKL	ubiquitination	[1]
106	GKICDQLKGHLKANT	acetylation	[2]
106	GKICDQLKGHLKANT	ubiquitination	[1]
110	DQLKGHLKANTIGIS	ubiquitination	[1]
120	TIGISLIKGVDEGPN	ubiquitination	[1]
130	DEGPNGLKLISEVIG	acetylation	[2]
130	DEGPNGLKLISEVIG	ubiquitination	[1]
169	CETTIGCKDPAQGQL	ubiquitination	[1]
178	PAQGQLLKDLMQTPN	ubiquitination	[1]
272	CYGGRNRKVAEAFAR	ubiquitination	[1]
282	EAFARTGKSIEQLEK	acetylation	[2]
289	KSIEQLEKEMLNGQK	acetylation	[2]
289	KSIEQLEKEMLNGQK	ubiquitination	[1]
296	KEMLNGQKLQGPQTA	ubiquitination	[1]
313	LHSILQHKGLVDKFP	ubiquitination	[1]
318	QHKGLVDKFPLFTAV	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

  

Sequence Annotation

 NP_BIND 10 15 NAD (By similarity).
 REGION 269 270 Substrate binding (By similarity).
 ACT_SITE 204 204 Proton acceptor (Potential).
 BINDING 41 41 NAD (By similarity).
 BINDING 97 97 NAD (By similarity).
 BINDING 120 120 Substrate (By similarity).
 BINDING 153 153 NAD; via amide nitrogen (By similarity).
 BINDING 269 269 NAD (By similarity).
 BINDING 296 296 NAD; via amide nitrogen (By similarity).
 BINDING 298 298 NAD (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 349 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; TAS:MGI.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IEA:EC.
 GO:0004368; F:glycerol-3-phosphate dehydrogenase activity; IDA:MGI.
 GO:0051287; F:NAD binding; IEA:Compara.
 GO:0071320; P:cellular response to cAMP; IDA:MGI.
 GO:0071356; P:cellular response to tumor necrosis factor; IDA:MGI.
 GO:0006094; P:gluconeogenesis; IMP:MGI.
 GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
 GO:0006072; P:glycerol-3-phosphate metabolic process; IMP:MGI.
 GO:0046486; P:glycerolipid metabolic process; IEA:Compara.
 GO:0006116; P:NADH oxidation; IEA:Compara. 

Interpro

 IPR008927; 6-PGluconate_DH_C-like.
 IPR013328; DH_multihelical.
 IPR006168; G3P_DH_NAD-dep.
 IPR006109; G3P_DH_NAD-dep_C.
 IPR017751; G3P_DH_NAD-dep_euk.
 IPR011128; G3P_DH_NAD-dep_N.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF07479; NAD_Gly3P_dh_C
 PF01210; NAD_Gly3P_dh_N 

SMART

  

PROSITE

 PS00957; NAD_G3PDH 

PRINTS

 PR00077; GPDHDRGNASE.