CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010876
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor Sp1 
Protein Synonyms/Alias
  
Gene Name
 Sp1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
15MDEVTAVKIEKGVGGacetylation[1]
18VTAVKIEKGVGGNNGacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression (By similarity). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. 
Sequence Annotation
 ZN_FING 627 656 C2H2-type 1.
 ZN_FING 657 686 C2H2-type 2.
 ZN_FING 687 714 C2H2-type 3.
 REGION 2 83 Repressor domain (By similarity).
 REGION 147 252 Transactivation domain A (Gln-rich) (By
 REGION 262 496 Transactivation domain B (Gln-rich) (By
 REGION 497 611 Transactivation domain C (highly charged)
 REGION 620 786 VZV IE62-binding (By similarity).
 REGION 709 786 Domain D (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 2 2 Phosphoserine (By similarity).
 MOD_RES 7 7 Phosphoserine (By similarity).
 MOD_RES 60 60 Phosphoserine (By similarity).
 MOD_RES 102 102 Phosphoserine; by ATM (By similarity).
 MOD_RES 279 279 Phosphothreonine; by MAPK8 (By
 MOD_RES 454 454 Phosphothreonine; by MAPK1 AND MAPK3 (By
 MOD_RES 613 613 Phosphoserine; alternate (By similarity).
 MOD_RES 641 641 Phosphothreonine; alternate (By
 MOD_RES 642 642 Phosphoserine; by PKC/PRKCZ; alternate
 MOD_RES 652 652 Phosphothreonine; by PKC/PRKCZ (By
 MOD_RES 669 669 Phosphothreonine (By similarity).
 MOD_RES 671 671 Phosphoserine; by PKC/PRKCZ (By
 MOD_RES 682 682 Phosphothreonine; by PKC/PRKCZ (By
 MOD_RES 703 703 Phosphoserine; alternate (By similarity).
 MOD_RES 704 704 N6-acetyllysine (By similarity).
 MOD_RES 740 740 Phosphothreonine; by MAPK1, MAPK3 AND
 CARBOHYD 492 492 O-linked (GlcNAc) (By similarity).
 CARBOHYD 613 613 O-linked (GlcNAc); alternate (By
 CARBOHYD 641 641 O-linked (GlcNAc); alternate (By
 CARBOHYD 642 642 O-linked (GlcNAc); alternate (By
 CARBOHYD 699 699 O-linked (GlcNAc) (By similarity).
 CARBOHYD 703 703 O-linked (GlcNAc); alternate (By
 CROSSLNK 15 15 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Activator; Complete proteome; Cytoplasm; DNA-binding; Glycoprotein; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 786 AA 
Protein Sequence
MSDQDHSMDE VTAVKIEKGV GGNNGGSGNG GGAAFSQTRS SSTGSSSSSG GGGGQESQPS 60
PLALLAATCS RIESPNENSN NSQGPSQSGG TGELDLTATQ LSQGANGWQI ISSSSGATPT 120
SKEQSGNSTN GSNGSESSKN RTVSGGQYVV AATPNLQNQQ VLTGLPGVMP NIQYQVIPQF 180
QTVDGQQLQF AATGAQVQQD GSGQIQIIPG ANQQIITNRG SGGNIIAAMP NLLQQAVPLQ 240
GLANNVLSGQ TQYVTNVPVA LNGNITLLPV NSVSAATLTP SSQAGTISSS GSQESGSQPV 300
TSGTAISSAS LVSSQASSSS FFTNANSYST TTTTSNMGIM NFTSSGSSGT SSQGQTSQRV 360
GGLQGSDSLN IQQNQTSGGS LQGSQQKEGE QSQQTQQQQI LIQPQLVQGG QALQALQAAP 420
LSGQTFTTQA ISQETLQNLQ LQAVQNSGPI IIRTPTVGPN GQVSWQTLQL QNLQVQNPQA 480
QTITLAPMQG VSLGQTSSSN TTLTPIASAA SIPAGTVTVN AAQLSSMPGL QTINLSALGT 540
SGIQVHQLPG LPLAIANTPG DHGAQLGLHG PGGDGIHDET AGGEEGENSP DPQPQAGRRT 600
RREACTCPYC KDSEGRGSGD PGKKKQHICH IQGCGKVYGK TSHLRAHLRW HTGERPFMCN 660
WSYCGKRFTR SDELQRHKRT HTGEKKFACP ECPKRFMRSD HLSKHIKTHQ NKKGGPGVAL 720
SVGTLPLDSG AGSEGSGTAT PSALITTNMV AMEAICPEGI ARLANSGINV MQVTELQSIN 780
ISGNGF 786 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0032993; C:protein-DNA complex; IDA:RGD.
 GO:0000987; F:core promoter proximal region sequence-specific DNA binding; IEA:Compara.
 GO:0003690; F:double-stranded DNA binding; IDA:RGD.
 GO:0035326; F:enhancer binding; IEA:Compara.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IDA:RGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0060216; P:definitive hemopoiesis; IEA:Compara.
 GO:0048596; P:embryonic camera-type eye morphogenesis; IEA:Compara.
 GO:0001892; P:embryonic placenta development; IEA:Compara.
 GO:0060136; P:embryonic process involved in female pregnancy; IEA:Compara.
 GO:0048706; P:embryonic skeletal system development; IEA:Compara.
 GO:0043353; P:enucleate erythrocyte differentiation; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0030219; P:megakaryocyte differentiation; IEA:Compara.
 GO:0001503; P:ossification; IEA:Compara.
 GO:0043923; P:positive regulation by host of viral transcription; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IMP:RGD.
 GO:0001829; P:trophectodermal cell differentiation; IEA:Compara. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS