UniProt Accession

 AIRE_HUMAN; O43918; B2RP50; O43922; O43932; O75745 

Genbank Protein ID

 AB006682; AB006683; AB006684; AB006684; AB006684; AB006685; Z97990; AJ009610; AP001754; AP001060; CH471079; BC137268; BC137270 

Genbank Nucleotide ID

 BAA23988.1; BAA23989.1; BAA23990.1; BAA23991.1; BAA23992.1; BAA23993.1; CAB10790.1; CAA08759.1; BAA95560.1; EAX09443.1; AAI37269.1; AAI37271.1 

Protein Name

 Autoimmune regulator 

Protein Synonyms/Alias

 Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; APECED protein 

Gene Name

 AIRE 

Gene Synonyms/Alias

 APECED 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
114	SQPRKGRKPPAVPKA	acetylation	[1]
120	RKPPAVPKALVPPPR	acetylation	[1]
131	PPPRLPTKRKASEEA	acetylation	[1]
159	ASPGSQLKAKPPKKP	acetylation	[1]
164	QLKAKPPKKPESSAE	acetylation	[1]
165	LKAKPPKKPESSAEQ	acetylation	[1]
221	VFESGGSKKCIQVGG	acetylation	[1]
222	FESGGSKKCIQVGGE	acetylation	[1]
243	FEDSGSGKNKARSSS	acetylation	[1]
245	DSGSGKNKARSSSGP	acetylation	[1]
253	ARSSSGPKPLVRAKG	acetylation	[1]
259	PKPLVRAKGAQGAAP	acetylation	[1]

Reference

 [1] Autoimmune regulator is acetylated by transcription coactivator CBP/p300.
 Saare M, Rebane A, Rajashekar B, Vilo J, Peterson P.
 Exp Cell Res. 2012 Aug 15;318(14):1767-78. [PMID: 22659170] 

Functional Description

 Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions. 

Sequence Annotation

 DOMAIN 1 105 HSR.
 DOMAIN 181 280 SAND.
 ZN_FING 296 343 PHD-type 1.
 ZN_FING 434 475 PHD-type 2.
 REGION 295 298 Interaction with histone H3 not
 REGION 304 312 Interaction with histone H3 not
 REGION 331 335 Interaction with histone H3 not
 MOTIF 7 11 LXXLL motif 1.
 MOTIF 63 67 LXXLL motif 2.
 MOTIF 414 418 LXXLL motif 3.
 MOTIF 516 520 LXXLL motif 4.  

Keyword

 3D-structure; Activator; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 545 AA 

Protein Sequence

Gene Ontology

 GO:0015629; C:actin cytoskeleton; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0042393; F:histone binding; IDA:UniProtKB.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
 GO:0045182; F:translation regulator activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0006959; P:humoral immune response; IEA:Compara.
 GO:0006955; P:immune response; TAS:ProtInc.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006417; P:regulation of translation; IEA:GOC.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR008087; AIRE.
 IPR000770; SAND_dom.
 IPR010919; SAND_dom-like.
 IPR004865; Sp100.
 IPR019786; Zinc_finger_PHD-type_CS.
 IPR011011; Znf_FYVE_PHD.
 IPR001965; Znf_PHD.
 IPR019787; Znf_PHD-finger.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF00628; PHD
 PF01342; SAND
 PF03172; Sp100 

SMART

 SM00249; PHD
 SM00258; SAND 

PROSITE

 PS51414; HSR
 PS50864; SAND
 PS01359; ZF_PHD_1
 PS50016; ZF_PHD_2 

PRINTS

 PR01711; AIREGULATOR.