CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008366
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 28S ribosomal protein S29, mitochondrial 
Protein Synonyms/Alias
 MRP-S29; S29mt; Death-associated protein 3; DAP-3; Ionizing radiation resistance conferring protein 
Gene Name
 DAP3 
Gene Synonyms/Alias
 MRPS29 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
103EACLMVRKPALELLHubiquitination[1]
175LLQSSYNKQRFDQPLacetylation[2, 3]
175LLQSSYNKQRFDQPLubiquitination[1]
189LEASTWLKNFKTTNEubiquitination[1, 4, 5]
192STWLKNFKTTNERFLubiquitination[1]
203ERFLNQIKVQEKYVWubiquitination[1]
207NQIKVQEKYVWNKREacetylation[2, 6]
218NKRESTEKGSPLGEVubiquitination[1, 5, 7]
245DAVGIVLKELKRQSSubiquitination[1]
318SQTGSLFKPRKAYLPubiquitination[5]
377KAPTEEGKKELLFLSubiquitination[1]
378APTEEGKKELLFLSNubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Involved in mediating interferon-gamma-induced cell death. 
Sequence Annotation
 MOD_RES 175 175 N6-acetyllysine.
 MOD_RES 207 207 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Apoptosis; Complete proteome; Mitochondrion; Polymorphism; Reference proteome; Ribonucleoprotein; Ribosomal protein; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 398 AA 
Protein Sequence
MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG 60
DQHEGQHYNI SPQDLETVFP HGLPPRFVMQ VKTFSEACLM VRKPALELLH YLKNTSFAYP 120
AIRYLLYGEK GTGKTLSLCH VIHFCAKQDW LILHIPDAHL WVKNCRDLLQ SSYNKQRFDQ 180
PLEASTWLKN FKTTNERFLN QIKVQEKYVW NKRESTEKGS PLGEVVEQGI TRVRNATDAV 240
GIVLKELKRQ SSLGMFHLLV AVDGINALWG RTTLKREDKS PIAPEELALV HNLRKMMKND 300
WHGGAIVSAL SQTGSLFKPR KAYLPQELLG KEGFDALDPF IPILVSNYNP KEFESCIQYY 360
LENNWLQHEK APTEEGKKEL LFLSNANPSL LERHCAYL 398 
Gene Ontology
 GO:0005761; C:mitochondrial ribosome; IEA:InterPro.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0015935; C:small ribosomal subunit; IEA:InterPro.
 GO:0008637; P:apoptotic mitochondrial changes; IEA:Compara.
 GO:0097190; P:apoptotic signaling pathway; TAS:ProtInc. 
Interpro
 IPR019368; Ribosomal_S23/S29_mit.
 IPR008092; Ribosomal_S29_mit. 
Pfam
 PF10236; DAP3 
SMART
  
PROSITE
  
PRINTS
 PR01716; DEATHASSOCP3.