CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029389
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 HCG1983504, isoform CRA_f 
Protein Synonyms/Alias
 Tubulin beta-3 chain; cDNA FLJ16289 fis, clone OCBBF2009920, highly similar to TUBULIN BETA-3 CHAIN; cDNA FLJ77784 
Gene Name
 TUBB3 
Gene Synonyms/Alias
 hCG_1983504 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31GAGNNWAKGHYTEGAubiquitination[1]
144DICFRTLKLATPTYGubiquitination[1, 2]
225TQQMFDAKNMMAACDubiquitination[2, 3, 4, 5, 6, 7]
252FRGRMSMKEVDEQMLubiquitination[1, 2]
264QMLAIQSKNSSYFVEubiquitination[1, 2]
278EWIPNNVKVAVCDIPubiquitination[1, 2]
290DIPPRGLKMSSTFIGubiquitination[1, 3]
307TAIQELFKRISEQFTubiquitination[1, 3]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). 
Sequence Annotation
  
Keyword
 Complete proteome; GTP-binding; Microtubule; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 378 AA 
Protein Sequence
MDSVRSGAFG HLFRPDNFIF GQSGAGNNWA KGHYTEGAEL VDSVLDVVRK ECENCDCLQG 60
FQLTHSLGGG TGSGMGTLLI SKVREEYPDR IMNTFSVVPS PKVSDTVVEP YNATLSIHQL 120
VENTDETYCI DNEALYDICF RTLKLATPTY GDLNHLVSAT MSGVTTSLRF PGQLNADLRK 180
LAVNMVPFPR LHFFMPGFAP LTARGSQQYR ALTVPELTQQ MFDAKNMMAA CDPRHGRYLT 240
VATVFRGRMS MKEVDEQMLA IQSKNSSYFV EWIPNNVKVA VCDIPPRGLK MSSTFIGNST 300
AIQELFKRIS EQFTAMFRRK AFLHWYTGEG MDEMEFTEAE SNMNDLVSEY QQYQDATAEE 360
EGEMYEDDEE ESEAQGPK 378 
Gene Ontology
 GO:0030424; C:axon; IEA:Compara.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0042277; F:peptide binding; IEA:Compara.
 GO:0005200; F:structural constituent of cytoskeleton; IEA:InterPro.
 GO:0007411; P:axon guidance; IEA:Compara.
 GO:0007017; P:microtubule-based process; IEA:InterPro.
 GO:0007067; P:mitosis; IEA:Compara.
 GO:0051258; P:protein polymerization; IEA:InterPro. 
Interpro
 IPR002453; Beta_tubulin.
 IPR008280; Tub_FtsZ_C.
 IPR000217; Tubulin.
 IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
 IPR023123; Tubulin_C.
 IPR017975; Tubulin_CS.
 IPR003008; Tubulin_FtsZ_GTPase. 
Pfam
 PF00091; Tubulin
 PF03953; Tubulin_C 
SMART
 SM00864; Tubulin
 SM00865; Tubulin_C 
PROSITE
 PS00227; TUBULIN 
PRINTS
 PR01163; BETATUBULIN.
 PR01161; TUBULIN.