CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018861
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho guanine nucleotide exchange factor 1 
Protein Synonyms/Alias
 115 kDa guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; Sub1.5 
Gene Name
 ARHGEF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
477AEGSWFQKISSRFCSubiquitination[1]
534TEMQRLTKYPLLLQSubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation. 
Sequence Annotation
 DOMAIN 41 232 RGSL.
 DOMAIN 416 605 DH.
 DOMAIN 647 760 PH.
 MOD_RES 738 738 Phosphotyrosine; by JAK2.
 MOD_RES 863 863 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; GTPase activation; Guanine-nucleotide releasing factor; Membrane; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 912 AA 
Protein Sequence
MEDFARGAAS PGPSRPGLVP VSIIGAEDED FENELETNSE EQNSQFQSLE QVKRRPAHLM 60
ALLQHVALQF EPGPLVLRVP VPPNVAFELD RTRADLISED VQRRFVQEVV QSQQVAVGRQ 120
LEDFRSKRLM GMTPWEQELA QLEAWVGRDR ASYEARERHV AERLLMHLEE MQHTISTDEE 180
KSAAVVNAIG LYMRHLGVRT KSGDKKSGRN FFRKKVMGNR RSDEPAKTKK GLSSILDAAR 240
WNRGEPQVPD FRHLKAEVDA EKPGATDRKG GVGMPSRDRN IGAPGQDTPG VSLHPLSLDS 300
PDREPGADAP LELGDSSPQG PMSLESLAPP ESTDEGAETE SPEPGDEGEP GRSGLELEPE 360
EPPGWRELVP PDTLHSLPKS QVKRQEVISE LLVTEAAHVR MLRVLHDLFF QPMAECLFFP 420
LEELQNIFPS LDELIEVHSL FLDRLMKRRQ ESGYLIEEIG DVLLARFDGA EGSWFQKISS 480
RFCSRQSFAL EQLKAKQRKD PRFCAFVQEA ESRPRCRRLQ LKDMIPTEMQ RLTKYPLLLQ 540
SIGQNTEEPT EREKVELAAE CCREILHHVN QAVRDMEDLL RLKDYQRRLD LSHLRQSSDP 600
MLSEFKNLDI TKKKLVHEGP LTWRVTKDKA VEVHVLLLDD LLLLLQRQDE RLLLKSHSRT 660
LTPTPDGKTM LRPVLRLTSA MTREVATDHK AFYVLFTWDQ EAQIYELVAQ TVSERKNWCA 720
LITETAGSLK VPAPASRPKP RPSPSSTREP LLSSSENGNG GRETSPADAR TERILSDLLP 780
FCRPGPEGQL AATALRKVLS LKQLLFPAEE DNGAGPPRDG DGVPGGGPLS PARTQEIQEN 840
LLSLEETMKQ LEELEEEFCR LRPLLSQLGG NSVPQPGCT 879 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; TAS:ProtInc.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0050771; P:negative regulation of axonogenesis; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0007266; P:Rho protein signal transduction; TAS:ProtInc.
 GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR000219; DH-domain.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR015212; Regulat_G_prot_signal-like.
 IPR016137; Regulat_G_prot_signal_superfam.
 IPR015721; RhoGEF-like. 
Pfam
 PF09128; RGS-like
 PF00621; RhoGEF 
SMART
 SM00233; PH
 SM00325; RhoGEF 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN 
PRINTS