CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006478
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Prostaglandin E2 receptor EP4 subtype 
Protein Synonyms/Alias
 PGE receptor EP4 subtype; PGE2 receptor EP4 subtype; Prostanoid EP4 receptor 
Gene Name
 PTGER4 
Gene Synonyms/Alias
 PTGER2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
308SLEREVSKNPDLQAIubiquitination[1]
468GRAGPAPKGSSLQVTubiquitination[1, 2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. 
Sequence Annotation
 MOD_RES 374 374 Phosphoserine.
 MOD_RES 377 377 Phosphoserine.
 MOD_RES 379 379 Phosphoserine.
 MOD_RES 382 382 Phosphoserine.
 CARBOHYD 7 7 N-linked (GlcNAc...) (Potential).
 DISULFID 92 170 By similarity.  
Keyword
 Cell membrane; Complete proteome; Disulfide bond; G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein; Receptor; Reference proteome; Transducer; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 488 AA 
Protein Sequence
MSTPGVNSSA SLSPDRLNSP VTIPAVMFIF GVVGNLVAIV VLCKSRKEQK ETTFYTLVCG 60
LAVTDLLGTL LVSPVTIATY MKGQWPGGQP LCEYSTFILL FFSLSGLSII CAMSVERYLA 120
INHAYFYSHY VDKRLAGLTL FAVYASNVLF CALPNMGLGS SRLQYPDTWC FIDWTTNVTA 180
HAAYSYMYAG FSSFLILATV LCNVLVCGAL LRMHRQFMRR TSLGTEQHHA AAAASVASRG 240
HPAASPALPR LSDFRRRRSF RRIAGAEIQM VILLIATSLV VLICSIPLVV RVFVNQLYQP 300
SLEREVSKNP DLQAIRIASV NPILDPWIYI LLRKTVLSKA IEKIKCLFCR IGGSRRERSG 360
QHCSDSQRTS SAMSGHSRSF ISRELKEISS TSQTLLPDLS LPDLSENGLG GRNLLPGVPG 420
MGLAQEDTTS LRTLRISETS DSSQGQDSES VLLVDEAGGS GRAGPAPKGS SLQVTFPSET 480
LNLSEKCI 488 
Gene Ontology
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0004957; F:prostaglandin E receptor activity; IDA:UniProtKB.
 GO:0007188; P:adenylate cyclase-modulating G-protein coupled receptor signaling pathway; IDA:UniProtKB.
 GO:0060348; P:bone development; ISS:UniProtKB.
 GO:0071260; P:cellular response to mechanical stimulus; ISS:UniProtKB.
 GO:0070371; P:ERK1 and ERK2 cascade; ISS:UniProtKB.
 GO:0007254; P:JNK cascade; ISS:UniProtKB.
 GO:0050710; P:negative regulation of cytokine secretion; ISS:UniProtKB.
 GO:2000420; P:negative regulation of eosinophil extravasation; IDA:UniProtKB.
 GO:0050728; P:negative regulation of inflammatory response; IDA:UniProtKB.
 GO:0033624; P:negative regulation of integrin activation; IDA:UniProtKB.
 GO:0050715; P:positive regulation of cytokine secretion; ISS:UniProtKB.
 GO:0050729; P:positive regulation of inflammatory response; ISS:UniProtKB.
 GO:0030278; P:regulation of ossification; IEA:Compara.
 GO:0051492; P:regulation of stress fiber assembly; ISS:UniProtKB.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
 GO:0042093; P:T-helper cell differentiation; ISS:UniProtKB. 
Interpro
 IPR000276; GPCR_Rhodpsn.
 IPR017452; GPCR_Rhodpsn_7TM.
 IPR001758; Prost_EP4_rcpt.
 IPR008365; Prostanoid_rcpt.
 IPR001244; Prostglndn_DP_rcpt. 
Pfam
 PF00001; 7tm_1 
SMART
  
PROSITE
 PS00237; G_PROTEIN_RECEP_F1_1
 PS50262; G_PROTEIN_RECEP_F1_2 
PRINTS
 PR00237; GPCRRHODOPSN.
 PR00428; PROSTAGLNDNR.
 PR01788; PROSTANOIDR.
 PR00586; PRSTNOIDEP4R.