UniProt Accession

 DNAJ_ECOLI; P08622 

Genbank Protein ID

 M12544; M12565; U00096; AP009048 

Genbank Nucleotide ID

 AAA00009.1; AAA23693.1; AAC73126.1; BAB96590.1 

Protein Name

 Chaperone protein DnaJ 

Protein Synonyms/Alias

 HSP40; Heat shock protein J 

Gene Name

 dnaJ 

Gene Synonyms/Alias

 groP; b0015; JW0014 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
31	AYKRLAMKYHPDRNQ	acetylation	[1]
41	PDRNQGDKEAEAKFK	acetylation	[1]
46	GDKEAEAKFKEIKEA	acetylation	[1]
51	EAKFKEIKEAYEVLT	acetylation	[1]
62	EVLTDSQKRAAYDQY	acetylation	[1]
194	QGRGTLIKDPCNKCH	acetylation	[2]
215	RSKTLSVKIPAGVDT	acetylation	[1]
292	LDGRVKLKVPGETQT	acetylation	[1]
301	PGETQTGKLFRMRGK	acetylation	[1]
361	EHNSPRSKSFFDGVK	acetylation	[1]
368	KSFFDGVKKFFDDLT	acetylation	[1]
369	SFFDGVKKFFDDLTR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Interacts with DnaK and GrpE to disassemble a protein complex at the origins of replication of phage lambda and several plasmids. Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. 

Sequence Annotation

 DOMAIN 3 72 J.
 REPEAT 144 151 CXXCXGXG motif.
 REPEAT 161 168 CXXCXGXG motif.
 REPEAT 183 190 CXXCXGXG motif.
 REPEAT 197 204 CXXCXGXG motif.
 ZN_FING 131 209 CR-type.
 METAL 144 144 Zinc 1.
 METAL 147 147 Zinc 1.
 METAL 161 161 Zinc 2.
 METAL 164 164 Zinc 2.
 METAL 183 183 Zinc 2.
 METAL 186 186 Zinc 2.
 METAL 197 197 Zinc 1.
 METAL 200 200 Zinc 1.  

Keyword

 3D-structure; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; DNA replication; Metal-binding; Reference proteome; Repeat; Stress response; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 376 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:EcoliWiki.
 GO:0016020; C:membrane; IDA:EcoliWiki.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0003756; F:protein disulfide isomerase activity; IDA:EcoliWiki.
 GO:0051082; F:unfolded protein binding; IDA:EcoliWiki.
 GO:0008270; F:zinc ion binding; IDA:EcoliWiki.
 GO:0006260; P:DNA replication; IMP:EcoliWiki.
 GO:0042026; P:protein refolding; IDA:EcoliWiki.
 GO:0043335; P:protein unfolding; IDA:EcoCyc.
 GO:0009408; P:response to heat; IEP:EcoliWiki. 

Interpro

 IPR012724; DnaJ.
 IPR002939; DnaJ_C.
 IPR001623; DnaJ_domain.
 IPR018253; DnaJ_domain_CS.
 IPR008971; HSP40/DnaJ_pept-bd.
 IPR001305; HSP_DnaJ_Cys-rich_dom. 

Pfam

 PF00226; DnaJ
 PF01556; DnaJ_C
 PF00684; DnaJ_CXXCXGXG 

SMART

 SM00271; DnaJ 

PROSITE

 PS00636; DNAJ_1
 PS50076; DNAJ_2
 PS51188; ZF_CR 

PRINTS

 PR00625; JDOMAIN.