UniProt Accession

 CENPN_HUMAN; Q96H22; A8MZE6; B3KN53; B4DJD1; B4DPY7; C9JJM5; D3DUK8; E7ES30; E7ETS3; Q9NZ83 

Genbank Protein ID

 AF217515; AK023669; AK026313; AK298554; AK296024; AC092718; CH471114; CH471114; BC007334; BC008972 

Genbank Nucleotide ID

 AAF67626.1; BAG51215.1; BAG60749.1; BAG58793.1; EAW95553.1; EAW95556.1; AAH07334.1; AAH08972.1 

Protein Name

 Centromere protein N 

Protein Synonyms/Alias

 CENP-N; Interphase centromere complex protein 32 

Gene Name

 CENPN 

Gene Synonyms/Alias

 C16orf60; ICEN32; BM-309 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	ETVAEFIKRTILKIP	ubiquitination	[1]
15	FIKRTILKIPMNELT	ubiquitination	[1, 2]
90	WEVFQMSKGPGEDVD	ubiquitination	[2]
102	DVDLFDMKQFKNSFK	ubiquitination	[1, 2]
117	KILQRALKNVTVSFR	ubiquitination	[1, 2]
202	SRYLDSLKAIVFKQY	ubiquitination	[1, 2, 3]
207	SLKAIVFKQYNQTFE	ubiquitination	[4, 5]
245	IHENIVEKERVQRIT	ubiquitination	[1, 2, 6]
270	QLEFAQYKLETKFKS	ubiquitination	[2, 3]
274	AQYKLETKFKSGLNG	ubiquitination	[1]
276	YKLETKFKSGLNGSI	ubiquitination	[1, 4, 5]
296	EPLRCLIKFSSPHLL	ubiquitination	[1]
329	LLTCIPNKRMNYFKI	ubiquitination	[1, 6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. 

Sequence Annotation

 MOD_RES 226 226 Phosphoserine.
 MOD_RES 282 282 Phosphoserine.  

Keyword

 Alternative splicing; Centromere; Chromosome; Complete proteome; Kinetochore; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 339 AA 

Protein Sequence

Gene Ontology

 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0034080; P:CENP-A containing nucleosome assembly at centromere; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome. 

Interpro

 IPR007902; CHL4. 

Pfam

 PF05238; CENP-N 

SMART

  

PROSITE

  

PRINTS