CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004223
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein SNA3 
Protein Synonyms/Alias
  
Gene Name
 SNA3 
Gene Synonyms/Alias
 YJL151C; J0630 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
125DVPLMDNKQQLSSGRubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
Reference
 [1] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [2] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [3] A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking.
 Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
 Mol Cell Proteomics. 2006 Apr;5(4):737-48. [PMID: 16432255]
 [4] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [5] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [6] Structure and function of a HECT domain ubiquitin-binding site.
 Kim HC, Steffen AM, Oldham ML, Chen J, Huibregtse JM.
 EMBO Rep. 2011 Apr;12(4):334-41. [PMID: 21399621]
 [7] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [8] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301
Functional Description
  
Sequence Annotation
 CROSSLNK 125 125 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Cytoplasmic vesicle; Endosome; Isopeptide bond; Membrane; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 133 AA 
Protein Sequence
MDRDHINDHD HRMSYSINKD DLLLMVLAVF IPPVAVWKRK GMFNRDTLLN LLLFLLLFFP 60
AIIHACYVVY ETSSERSYDL SRRHATAPAV DRDLEAHPAE ESQAQPPAYD EDDEAGADVP 120
LMDNKQQLSS GRT 133 
Gene Ontology
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0000328; C:fungal-type vacuole lumen; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0031625; F:ubiquitin protein ligase binding; IMP:SGD.
 GO:0044257; P:cellular protein catabolic process; IMP:SGD.
 GO:0032509; P:endosome transport via multivesicular body sorting pathway; IMP:SGD. 
Interpro
 IPR000612; PMP3. 
Pfam
 PF01679; Pmp3 
SMART
  
PROSITE
 PS01309; UPF0057 
PRINTS