CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007538
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Forkhead box protein K1 
Protein Synonyms/Alias
 Myocyte nuclear factor; MNF 
Gene Name
 Foxk1 
Gene Synonyms/Alias
 Mnf 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
361RSQEEPGKGSFWRIDacetylation[1]
374IDPASEAKLVEQAFRubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli. 
Sequence Annotation
 DOMAIN 109 161 FHA.
 DNA_BIND 291 386 Fork-head.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 87 87 Phosphoserine (By similarity).
 MOD_RES 199 199 Phosphoserine.
 MOD_RES 209 209 Phosphoserine (By similarity).
 MOD_RES 225 225 Phosphoserine (By similarity).
 MOD_RES 229 229 Phosphoserine (By similarity).
 MOD_RES 239 239 Phosphoserine (By similarity).
 MOD_RES 243 243 Phosphoserine (By similarity).
 MOD_RES 285 285 Phosphoserine (By similarity).
 MOD_RES 402 402 Phosphoserine.
 MOD_RES 406 406 Phosphoserine.
 MOD_RES 414 414 Phosphoserine.
 MOD_RES 422 422 Phosphothreonine.
 MOD_RES 427 427 Phosphoserine (By similarity).
 MOD_RES 431 431 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Activator; Complete proteome; Developmental protein; Differentiation; DNA-binding; Myogenesis; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 719 AA 
Protein Sequence
MAEVGEDSGA RALLALRSAP CSPVLCAAAA AAAFPATTSP PPPAQPPPGP PALPAEPGPG 60
PVPSTVATAT TTAPALVAAA AASVRQSPGP ALARLEGREF EFLMRQPSVT IGRNSSQGSV 120
DLSMGLSSFI SRRHLQLSFQ EPHFYLRCLG KNGVFVDGAF QRRGAPALQL PQQCTFRFPS 180
TAIKIQFTSL YHKEEAPASP LRPLYPQISP LKIHIPEPDL RSLVSPIPSP TGTISVPNSC 240
PASPRGAGSS SYRFVQNVTS DLQLAAEFAA KAASEQQADA SGGDSPKDES KPPYSYAQLI 300
VQAISSAQDR QLTLSGIYAH ITKHYPYYRT ADKGWQNSIR HNLSLNRYFI KVPRSQEEPG 360
KGSFWRIDPA SEAKLVEQAF RKRRQRGVSC FRTPFGPLSS RSAPASPTHP GLMSPRSSGL 420
QTPECLSREG SPIPHDPDLG SKLASVPEYR YSQSAPGSPV SAQPVIMAVP PRPSNLVAKP 480
VAYMPASIVT SQQPSGHAIH VVQQAPTVTM VRVVTTSANS ANGYILASQG STGTSHDTAG 540
TAVLDLGNEA RGLEEKPTIA FATIPAASRV IQTVASQMAP GVPGHTVTIL QPATPVTIGQ 600
HHLPVRAVTQ NGKHAVPTNS LTGNAYALSS PLQLLAAQAS SSTPVVITRV CEVGPEEPAA 660
AVSVAANAAP TPAASTTTSA SSSGEPEVKR SRVEEPGGTA TTQPTAMAAT GPQGPGTGE 719 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005667; C:transcription factor complex; IBA:RefGenome.
 GO:0003677; F:DNA binding; IDA:UniProtKB.
 GO:0008301; F:DNA binding, bending; IBA:RefGenome.
 GO:0003690; F:double-stranded DNA binding; IBA:RefGenome.
 GO:0000287; F:magnesium ion binding; IBA:RefGenome.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IBA:RefGenome.
 GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
 GO:0008134; F:transcription factor binding; IBA:RefGenome.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0009790; P:embryo development; IBA:RefGenome.
 GO:0007517; P:muscle organ development; IEA:UniProtKB-KW.
 GO:0045786; P:negative regulation of cell cycle; TAS:UniProtKB.
 GO:0030308; P:negative regulation of cell growth; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IBA:RefGenome.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0007389; P:pattern specification process; IBA:RefGenome.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0051090; P:regulation of sequence-specific DNA binding transcription factor activity; IBA:RefGenome.
 GO:0009888; P:tissue development; IBA:RefGenome. 
Interpro
 IPR000253; FHA_dom.
 IPR008984; SMAD_FHA_domain.
 IPR001766; TF_fork_head.
 IPR018122; TF_fork_head_CS.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00498; FHA
 PF00250; Fork_head 
SMART
 SM00339; FH
 SM00240; FHA 
PROSITE
 PS50006; FHA_DOMAIN
 PS00657; FORK_HEAD_1
 PS00658; FORK_HEAD_2
 PS50039; FORK_HEAD_3 
PRINTS
 PR00053; FORKHEAD.