CPLM-012751

Genbank Nucleotide ID

V-type proton ATPase subunit F

Protein Synonyms/Alias

V-ATPase subunit F; V-ATPase 14 kDa subunit; Vacuolar proton pump subunit F

Homo sapiens (Human)

Position	Peptide	Type	References
94	AVLEIPSKEHPYDAA	ubiquitination	[1, 2, 3, 4, 5, 6]
102	EHPYDAAKDSILRRA	ubiquitination	[1, 5, 6, 7, 8]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Alternative splicing; Complete proteome; Hydrogen ion transport; Ion transport; Polymorphism; Reference proteome; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0033180; C:proton-transporting V-type ATPase, V1 domain; IEA:InterPro.
GO:0016471; C:vacuolar proton-transporting V-type ATPase complex; IDA:UniProtKB.
GO:0042624; F:ATPase activity, uncoupled; NAS:UniProtKB.
GO:0015078; F:hydrogen ion transmembrane transporter activity; NAS:UniProtKB.
GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IEA:InterPro.
GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro.
GO:0006879; P:cellular iron ion homeostasis; TAS:Reactome.
GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
GO:0051701; P:interaction with host; TAS:Reactome.
GO:0090382; P:phagosome maturation; TAS:Reactome.
GO:0033572; P:transferrin transport; TAS:Reactome.

IPR008218; ATPase_V1-cplx_f_g_su.
IPR005772; ATPase_V1-cplx_fsu_euk.