CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018582
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 
Protein Synonyms/Alias
 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Ribophorin I; RPN-I; Ribophorin-1 
Gene Name
 Rpn1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
117SGRFFTVKLPVALDPacetylation[1]
188RNVESYTKLGNPSRSacetylation[2, 3, 4]
188RNVESYTKLGNPSRSsuccinylation[3]
489QVLTLVNKRLGLYRHubiquitination[5]
525KSLETEHKAVTSEIAacetylation[6]
525KSLETEHKAVTSEIAubiquitination[5]
539AVLQSRLKTEGSDLCacetylation[1, 2, 3, 4, 7, 8]
539AVLQSRLKTEGSDLCubiquitination[5]
554DRVSEMQKLDAQVKEacetylation[7]
554DRVSEMQKLDAQVKEubiquitination[5]
560QKLDAQVKELVLKSAubiquitination[5]
565QVKELVLKSAVEAERubiquitination[5]
579RLVAGKLKKDTYLENubiquitination[5]
580LVAGKLKKDTYLENEubiquitination[5]
588DTYLENEKLSSGKRQacetylation[1, 3]
588DTYLENEKLSSGKRQubiquitination[5]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [8] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 
Functional Description
 Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains (By similarity). 
Sequence Annotation
 MOD_RES 188 188 N6-acetyllysine (By similarity).
 CARBOHYD 300 300 N-linked (GlcNAc...) (Potential).  
Keyword
 Acetylation; Complete proteome; Endoplasmic reticulum; Glycoprotein; Glycosyltransferase; Membrane; Reference proteome; Signal; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 608 AA 
Protein Sequence
MESPVALLLL LLLCLGALAP TPGSASSEAP PLVNEDVKRT VDLSSHLAKV TAEVVLVHPG 60
GGSTSRASSF VLALEPELES RLAHLGVQIK GEDEEDNNLE VRETKIKGKS GRFFTVKLPV 120
ALDPGSKISV VVETVYTHVL HPYPTQITQS EKQFVVFEGN HYFYSPYPTK TQTMRVKLAS 180
RNVESYTKLG NPSRSEDVLD YGPFKDIPAY SQDTFKVHYE NNSPFLTITS MTRVIEVSHW 240
GNIAVEENVD LKHTGAVLKG PFSRYDYQRQ PDSGISSIRS FKTILPAAAQ DVYYRDEIGN 300
VSTSHLLILD DSVEMEIRPR FPLFGGWKTH YIVGYNLPSY EYLYNLGDQY ALKMRFVDHV 360
FDEQVIDSLT VKIILPEGAK NIQVDSPYDI SRAPDELHYT YLDTFGRPVI VAYKKNLVEQ 420
HIQDIVVHYT FNKVLMLQEP LLVVAAFYIL FFTVIIYVRL DFSITKDPAA EARMKVACIT 480
EQVLTLVNKR LGLYRHFDET VNRYKQSRDI STLNSGKKSL ETEHKAVTSE IAVLQSRLKT 540
EGSDLCDRVS EMQKLDAQVK ELVLKSAVEA ERLVAGKLKK DTYLENEKLS SGKRQELVTK 600
IDHILDAL 608 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IDA:MGI.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0004579; F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; IEA:InterPro.
 GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway. 
Interpro
 IPR007676; Ribophorin_I. 
Pfam
 PF04597; Ribophorin_I 
SMART
  
PROSITE
  
PRINTS