CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009046
UniProt Accession
Genbank Protein ID
 U76557 
Genbank Nucleotide ID
Protein Name
 UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit 
Protein Synonyms/Alias
 O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT 
Gene Name
 Ogt 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
540YEHPKDLKLSDGRLRubiquitination[1]
1000SSPLFNTKQYTMELEubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta- linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O- GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues (By similarity). 
Sequence Annotation
 REPEAT 11 44 TPR 1.
 REPEAT 79 112 TPR 2.
 REPEAT 113 146 TPR 3.
 REPEAT 147 180 TPR 4.
 REPEAT 181 214 TPR 5.
 REPEAT 215 248 TPR 6.
 REPEAT 249 282 TPR 7.
 REPEAT 283 316 TPR 8.
 REPEAT 317 350 TPR 9.
 REPEAT 351 384 TPR 10.
 REPEAT 385 418 TPR 11.
 REPEAT 419 452 TPR 12.
 REPEAT 453 463 TPR 13; truncated.
 NP_BIND 895 898 UDP (By similarity).
 NP_BIND 901 904 UDP (By similarity).
 NP_BIND 919 921 UDP (By similarity).
 REGION 981 1000 Required for phosphatidylinositol 3,4,5-
 MOTIF 478 493 Nuclear localization signal (Potential).
 ACT_SITE 498 498 Proton acceptor (By similarity).
 BINDING 839 839 UDP (By similarity).
 BINDING 842 842 UDP (By similarity).
 BINDING 925 925 UDP (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 979 979 Phosphotyrosine (Probable).  
Keyword
 Acetylation; Cell membrane; Chromatin regulator; Complete proteome; Cytoplasm; Direct protein sequencing; Glycoprotein; Glycosyltransferase; Lipid-binding; Membrane; Nucleus; Phosphoprotein; Reference proteome; Repeat; TPR repeat; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1036 AA 
Protein Sequence
MASSVGNVAD STGLAELAHR EYQAGDFEAA ERHCMQLWRQ EPDNTGVLLL LSSIHFQCRR 60
LDRSAHFSTL AIKQNPLLAE AYSNLGNVYK ERGQLQEAIE HYRHALRLKP DFIDGYINLA 120
AALVAAGDME GAVQAYVSAL QYNPDLYCVR SDLGNLLKAL GRLEEAKACY LKAIETQPNF 180
AVAWSNLGCV FNAQGEIWLA IHHFEKAVTL DPNFLDAYIN LGNVLKEARI FDRAVAAYLR 240
ALSLSPNHAV VHGNLACVYY EQGLIDLAID TYRRAIELQP HFPDAYCNLA NALKEKGSVA 300
EAEDCYNTAL RLCPTHADSL NNLANIKREQ GNIEEAVRLY RKALEVFPEF AAAHSNLASV 360
LQQQGKLQEA LMHYKEAIRI SPTFADAYSN MGNTLKEMQD VQGALQCYTR AIQINPAFAD 420
AHSNLASIHK DSGNIPEAIA SYRTALKLKP DFPDAYCNLA HCLQIVCDWT DYDERMKKLV 480
SIVAEQLEKN RLPSVHPHHS MLYPLSHGFR KAIAERHGNL CLDKINVLHK PPYEHPKDLK 540
LSDGRLRVGY VSSDFGNHPT SHLMQSIPGM HNPDKFEVFC YALSPDDGTN FRVKVMAEAN 600
HFIDLSQIPC NGKAADRIHQ DGIHILVNMN GYTKGARNEL FALRPAPIQA MWLGYPGTSG 660
ALFMDYIITD QETSPAEVAE QYSEKLAYMP HTFFIGDHAN MFPHLKKKAV IDFKSNGHIY 720
DNRIVLNGID LKAFLDSLPD VKIVKMKCPD GGDNADTTNT ALNMPVIPMN TIAEAVIEMI 780
NRGQIQITIN GFSISNGLAT TQINNKAATG EEVPRTIIVT TRSQYGLPED AIVYCNFNQL 840
YKIDPSTLQM GANILKRVPN SVLWLLRFPA VGEPNIQQYA QNMGLPQNRI IFSPVAPKEE 900
HVRRGQLADV CLDTPLCNGH TTGMDVLWAG TPMVTMPGET LASRVAASQL TCLGCLELIA 960
KSRQEYEDIA VKLGTDLEYL KKIRGKVWKQ RISSPLFNTK QYTMELERLY LQMWEHYAAG 1020
NKPDHMIKPV EVTESA 1036 
Gene Ontology
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0000123; C:histone acetyltransferase complex; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0048029; F:monosaccharide binding; IDA:RGD.
 GO:0042277; F:peptide binding; IDA:RGD.
 GO:0005547; F:phosphatidylinositol-3,4,5-trisphosphate binding; ISS:UniProtKB.
 GO:0016262; F:protein N-acetylglucosaminyltransferase activity; IDA:RGD.
 GO:0080182; P:histone H3-K4 trimethylation; ISS:UniProtKB.
 GO:0043984; P:histone H4-K16 acetylation; ISS:UniProtKB.
 GO:0043981; P:histone H4-K5 acetylation; ISS:UniProtKB.
 GO:0043982; P:histone H4-K8 acetylation; ISS:UniProtKB.
 GO:0006917; P:induction of apoptosis; ISS:UniProtKB.
 GO:0048015; P:phosphatidylinositol-mediated signaling; ISS:UniProtKB.
 GO:0045862; P:positive regulation of proteolysis; ISS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0070207; P:protein homotrimerization; IDA:RGD.
 GO:0006493; P:protein O-linked glycosylation; IDA:RGD.
 GO:0006110; P:regulation of glycolysis; ISS:UniProtKB. 
Interpro
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS