CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012633
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial import receptor subunit TOM34 
Protein Synonyms/Alias
 hTom34; Translocase of outer membrane 34 kDa subunit 
Gene Name
 TOMM34 
Gene Synonyms/Alias
 URCC3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
63NRAACHLKDGNCRDCacetylation[1]
72GNCRDCIKDCTSALAubiquitination[1]
143LGPEWRLKLPSIPLVubiquitination[1]
278HKALKDYKSSFADISubiquitination[2, 3, 4, 5]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state. 
Sequence Annotation
 REPEAT 9 42 TPR 1.
 REPEAT 51 84 TPR 2.
 REPEAT 86 118 TPR 3.
 REPEAT 193 226 TPR 4.
 REPEAT 227 260 TPR 5.
 REPEAT 262 294 TPR 6.
 MOD_RES 186 186 Phosphoserine.  
Keyword
 Chaperone; Complete proteome; Cytoplasm; Membrane; Mitochondrion; Mitochondrion outer membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 309 AA 
Protein Sequence
MAPKFPDSVE ELRAAGNESF RNGQYAEASA LYGRALRVLQ AQGSSDPEEE SVLYSNRAAC 60
HLKDGNCRDC IKDCTSALAL VPFSIKPLLR RASAYEALEK YPMAYVDYKT VLQIDDNVTS 120
AVEGINRMTR ALMDSLGPEW RLKLPSIPLV PVSAQKRWNS LPSENHKEMA KSKSKETTAT 180
KNRVPSAGDV EKARVLKEEG NELVKKGNHK KAIEKYSESL LCSNLESATY SNRALCYLVL 240
KQYTEAVKDC TEALKLDGKN VKAFYRRAQA HKALKDYKSS FADISNLLQI EPRNGPAQKL 300
RQEVKQNLH 309 
Gene Ontology
 GO:0016021; C:integral to membrane; TAS:ProtInc.
 GO:0005741; C:mitochondrial outer membrane; IDA:UniProtKB.
 GO:0006626; P:protein targeting to mitochondrion; IMP:UniProtKB. 
Interpro
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR013105; TPR_2.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1
 PF07719; TPR_2 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS