UniProt Accession

 IDI1_HUMAN; Q13907; B4E155; Q32Q13; Q53GQ6; Q86U81; Q8WUX8; Q96IZ4; Q9BQ74 

Genbank Protein ID

 X17025; AF291755; AF271720; AF271724; AF271721; AF271722; AF271723; BT006761; AK303669; AC022536; CH471072; BC005247; BC006999; BC019227; BC022418; BC025375; BC057827; BC107893; AK222875 

Genbank Nucleotide ID

 CAA34890.1; AAK29357.1; AAK49435.1; AAK49434.1; AAK49434.1; AAK49434.1; AAK49434.1; AAP35407.1; BAG64667.1; EAW86521.1; AAH05247.2; AAH06999.2; AAH19227.2; AAH22418.2; AAH25375.2; AAH57827.1; AAI07894.1; BAD96595.1 

Protein Name

 Isopentenyl-diphosphate Delta-isomerase 1 

Protein Synonyms/Alias

 Isopentenyl pyrophosphate isomerase 1; IPP isomerase 1; IPPI1 

Gene Name

 IDI1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
94	KIGAETKKNCHLNEN	ubiquitination	[1]
104	HLNENIEKGLLHRAF	ubiquitination	[1, 2]
131	LQQRSDAKITFPGCF	ubiquitination	[1]
170	RAAQRRLKAELGIPL	ubiquitination	[1, 2]
214	DYILLVRKNVTLNPD	ubiquitination	[1]
226	NPDPNEIKSYCYVSK	ubiquitination	[2]
233	KSYCYVSKEELKELL	ubiquitination	[1]
237	YVSKEELKELLKKAA	ubiquitination	[2]
249	KAASGEIKITPWFKI	ubiquitination	[1, 2]
280	NQFVDHEKIYRM***	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). 

Sequence Annotation

 DOMAIN 49 199 Nudix hydrolase.
 MOTIF 225 227 Microbody targeting signal.
 ACT_SITE 86 86
 ACT_SITE 148 148
 METAL 40 40 Magnesium.
 METAL 51 51 Magnesium.
 METAL 146 146 Magnesium.
 METAL 148 148 Magnesium.
 BINDING 36 36 Substrate.
 BINDING 70 70 Substrate.
 BINDING 74 74 Substrate.
 BINDING 87 87 Substrate.
 MOD_RES 176 176 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Isomerase; Isoprene biosynthesis; Lipid biosynthesis; Lipid metabolism; Magnesium; Metal-binding; Peroxisome; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 227 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005777; C:peroxisome; IDA:UniProtKB.
 GO:0016787; F:hydrolase activity; IEA:InterPro.
 GO:0004452; F:isopentenyl-diphosphate delta-isomerase activity; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0030145; F:manganese ion binding; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006695; P:cholesterol biosynthetic process; TAS:Reactome.
 GO:0050992; P:dimethylallyl diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0008299; P:isoprenoid biosynthetic process; IDA:UniProtKB.
 GO:0035634; P:response to stilbenoid; IEA:Compara. 

Interpro

 IPR011876; IsopentenylPP_isomerase_typ1.
 IPR000086; NUDIX_hydrolase_dom.
 IPR015797; NUDIX_hydrolase_dom-like. 

Pfam

 PF00293; NUDIX 

SMART

  

PROSITE

 PS51462; NUDIX 

PRINTS