CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001792
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional aspartokinase/homoserine dehydrogenase 2 
Protein Synonyms/Alias
 Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase 
Gene Name
 metL 
Gene Synonyms/Alias
 metM; b3940; JW3911 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
16AKGRQLHKFGGSSLAacetylation[1]
26GSSLADVKCYLRVAGacetylation[1]
331VPASQDFKLAHKEIDacetylation[1]
335QDFKLAHKEIDQILKacetylation[1]
342KEIDQILKRAQVRPLacetylation[1]
582AGASDSNKYRQIHDAacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 NP_BIND 464 471 NADP (Potential).
 REGION 2 252 Aspartokinase.
 REGION 253 463 Interface.
 REGION 464 810 Homoserine dehydrogenase.  
Keyword
 Amino-acid biosynthesis; ATP-binding; Complete proteome; Direct protein sequencing; Kinase; Methionine biosynthesis; Multifunctional enzyme; NADP; Nucleotide-binding; Oxidoreductase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 810 AA 
Protein Sequence
MSVIAQAGAK GRQLHKFGGS SLADVKCYLR VAGIMAEYSQ PDDMMVVSAA GSTTNQLINW 60
LKLSQTDRLS AHQVQQTLRR YQCDLISGLL PAEEADSLIS AFVSDLERLA ALLDSGINDA 120
VYAEVVGHGE VWSARLMSAV LNQQGLPAAW LDAREFLRAE RAAQPQVDEG LSYPLLQQLL 180
VQHPGKRLVV TGFISRNNAG ETVLLGRNGS DYSATQIGAL AGVSRVTIWS DVAGVYSADP 240
RKVKDACLLP LLRLDEASEL ARLAAPVLHA RTLQPVSGSE IDLQLRCSYT PDQGSTRIER 300
VLASGTGARI VTSHDDVCLI EFQVPASQDF KLAHKEIDQI LKRAQVRPLA VGVHNDRQLL 360
QFCYTSEVAD SALKILDEAG LPGELRLRQG LALVAMVGAG VTRNPLHCHR FWQQLKGQPV 420
EFTWQSDDGI SLVAVLRTGP TESLIQGLHQ SVFRAEKRIG LVLFGKGNIG SRWLELFARE 480
QSTLSARTGF EFVLAGVVDS RRSLLSYDGL DASRALAFFN DEAVEQDEES LFLWMRAHPY 540
DDLVVLDVTA SQQLADQYLD FASHGFHVIS ANKLAGASDS NKYRQIHDAF EKTGRHWLYN 600
ATVGAGLPIN HTVRDLIDSG DTILSISGIF SGTLSWLFLQ FDGSVPFTEL VDQAWQQGLT 660
EPDPRDDLSG KDVMRKLVIL AREAGYNIEP DQVRVESLVP AHCEGGSIDH FFENGDELNE 720
QMVQRLEAAR EMGLVLRYVA RFDANGKARV GVEAVREDHP LASLLPCDNV FAIESRWYRD 780
NPLVIRGPGA GRDVTAGAIQ SDINRLAQLL 810 
Gene Ontology
 GO:0004072; F:aspartate kinase activity; IDA:EcoCyc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004412; F:homoserine dehydrogenase activity; IDA:EcoCyc.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0009090; P:homoserine biosynthetic process; IDA:EcoCyc.
 GO:0009089; P:lysine biosynthetic process via diaminopimelate; IDA:EcoCyc.
 GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
 GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR001048; Asp/Glu/Uridylate_kinase.
 IPR005106; Asp/hSer_DH_NAD-bd.
 IPR001341; Asp_kinase_dom.
 IPR018042; Aspartate_kinase_CS.
 IPR011147; Bifunc_aspartokin/hSer_DH.
 IPR001342; HDH_cat.
 IPR019811; HDH_CS.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00696; AA_kinase
 PF00742; Homoserine_dh
 PF03447; NAD_binding_3 
SMART
  
PROSITE
 PS00324; ASPARTOKINASE
 PS01042; HOMOSER_DHGENASE 
PRINTS