CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024448
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CCR4-NOT transcription complex subunit 1 
Protein Synonyms/Alias
 CCR4-associated factor 1; Negative regulator of transcription subunit 1 homolog; NOT1H; hNOT1 
Gene Name
 CNOT1 
Gene Synonyms/Alias
 CDC39; KIAA1007; NOT1; AD-005 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
556GEQYDQAKLSRILDVubiquitination[1]
2146DPFTPNLKVDMLSEIubiquitination[1]
2180KDLDSYLKTRSPVTFubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA- mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of emryonic stem (ES) cell identity. 
Sequence Annotation
 REGION 1090 1605 Interaction with CNOT6, CNOT6L, CNOT7 and
 MOTIF 153 157 LXXLL.
 MOTIF 181 185 LXXLL.
 MOTIF 223 227 LXXLL.
 MOTIF 570 574 LXXLL.
 MOTIF 1639 1643 LXXLL.
 MOTIF 1942 1946 LXXLL.
 MOTIF 2096 2100 LXXLL.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Nucleus; Polymorphism; Reference proteome; Repressor; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2376 AA 
Protein Sequence
MNLDSLSLAL SQISYLVDNL TKKNYRASQQ EIQHIVNRHG PEADRHLLRC LFSHVDFSGD 60
GKSSGKDFHQ TQFLIQECAL LITKPNFIST LSYAIDNPLH YQKSLKPAPH LFAQLSKVLK 120
LSKVQEVIFG LALLNSSSSD LRGFAAQFIK QKLPDLLRSY IDADVSGNQE GGFQDIAIEV 180
LHLLLSHLLF GQKGAFGVGQ EQIDAFLKTL RRDFPQERCP VVLAPLLYPE KRDILMDRIL 240
PDSGGVAKTM MESSLADFMQ EVGYGFCASI EECRNIIVQF GVREVTAAQV ARVLGMMART 300
HSGLTDGIPL QSISAPGSGI WSDGKDKSDG AQAHTWNVEV LIDVLKELNP SLNFKEVTYE 360
LDHPGFQIRD SKGLHNVVYG IQRGLGMEVF PVDLIYRPWK HAEGQLSFIQ HSLINPEIFC 420
FADYPCHTVA TDILKAPPED DNREIATWKS LDLIESLLRL AEVGQYEQVK QLFSFPIKHC 480
PDMLVLALLQ INTSWHTLRH ELISTLMPIF LGNHPNSAII LHYAWHGQGQ SPSIRQLIMH 540
AMAEWYMRGE QYDQAKLSRI LDVAQDLKAL SMLLNGTPFA FVIDLAALAS RREYLKLDKW 600
LTDKIREHGE PFIQACMTFL KRRCPSILGG LAPEKDQPKS AQLPPETLAT MLACLQACAG 660
SVSQELSETI LTMVANCSNV MNKARQPPPG VMPKGRPPSA SSLDAISPVQ IDPLAGMTSL 720
SIGGSAAPHT QSMQGFPPNL GSAFSTPQSP AKAFPPLSTP NQTTAFSGIG GLSSQLPVGG 780
LGTGSLTGIG TGALGLPAVN NDPFVQRKLG TSGLNQPTFQ QTDLSQVWPE ANQHFSKEID 840
DEANSYFQRI YNHPPHPTMS VDEVLEMLQR FKDSTIKRER EVFNCMLRNL FEEYRFFPQY 900
PDKELHITAC LFGGIIEKGL VTYMALGLAL RYVLEALRKP FGSKMYYFGI AALDRFKNRL 960
KDYPQYCQHL ASISHFMQFP HHLQEYIEYG QQSRDPPVKM QGSITTPGSI ALAQAQAQAQ 1020
VPAKAPLAGQ VSTMVTTSTT TTVAKTVTVT RPTGVSFKKD VPPSINTTNI DTLLVATDQT 1080
ERIVEPPENI QEKIAFIFNN LSQSNMTQKV EELKETVKEE FMPWVSQYLV MKRVSIEPNF 1140
HSLYSNFLDT LKNPEFNKMV LNETYRNIKV LLTSDKAAAN FSDRSLLKNL GHWLGMITLA 1200
KNKPILHTDL DVKSLLLEAY VKGQQELLYV VPFVAKVLES SIRSVVFRPP NPWTMAIMNV 1260
LAELHQEHDL KLNLKFEIEV LCKNLALDIN ELKPGNLLKD KDRLKNLDEQ LSAPKKDVKQ 1320
PEELPPITTT TTSTTPATNT TCTATVPPQP QYSYHDINVY SLAGLAPHIT LNPTIPLFQA 1380
HPQLKQCVRQ AIERAVQELV HPVVDRSIKI AMTTCEQIVR KDFALDSEES RMRIAAHHMM 1440
RNLTAGMAMI TCREPLLMSI STNLKNSFAS ALRTASPQQR EMMDQAAAQL AQDNCELACC 1500
FIQKTAVEKA GPEMDKRLAT EFELRKHARQ EGRRYCDPVV LTYQAERMPE QIRLKVGGVD 1560
PKQLAVYEEF ARNVPGFLPT NDLSQPTGFL AQPMKQAWAT DDVAQIYDKC ITELEQHLHA 1620
IPPTLAMNPQ AQALRSLLEV VVLSRNSRDA IAALGLLQKA VEGLLDATSG ADADLLLRYR 1680
ECHLLVLKAL QDGRAYGSPW CNKQITRCLI ECRDEYKYNV EAVELLIRNH LVNMQQYDLH 1740
LAQSMENGLN YMAVAFAMQL VKILLVDERS VAHVTEADLF HTIETLMRIN AHSRGNAPEG 1800
LPQLMEVVRS NYEAMIDRAH GGPNFMMHSG ISQASEYDDP PGLREKAEYL LREWVNLYHS 1860
AAAGRDSTKA FSAFVGQMHQ QGILKTDDLI TRFFRLCTEM CVEISYRAQA EQQHNPAANP 1920
TMIRAKCYHN LDAFVRLIAL LVKHSGEATN TVTKINLLNK VLGIVVGVLL QDHDVRQSEF 1980
QQLPYHRIFI MLLLELNAPE HVLETINFQT LTAFCNTFHI LRPTKAPGFV YAWLELISHR 2040
IFIARMLAHT PQQKGWPMYA QLLIDLFKYL APFLRNVELT KPMQILYKGT LRVLLVLLHD 2100
FPEFLCDYHY GFCDVIPPNC IQLRNLILSA FPRNMRLPDP FTPNLKVDML SEINIAPRIL 2160
TNFTGVMPPQ FKKDLDSYLK TRSPVTFLSD LRSNLQVSNE PGNRYNLQLI NALVLYVGTQ 2220
AIAHIHNKGS TPSMSTITHS AHMDIFQNLA VDLDTEGRYL FLNAIANQLR YPNSHTHYFS 2280
CTMLYLFAEA NTEAIQEQIT RVLLERLIVN RPHPWGLLIT FIELIKNPAF KFWNHEFVHC 2340
APEIEKLFQS VAQCCMGQKQ AQQVMEGTGA S 2371 
Gene Ontology
 GO:0030014; C:CCR4-NOT complex; IDA:UniProtKB.
 GO:0000932; C:cytoplasmic mRNA processing body; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005778; C:peroxisomal membrane; IDA:UniProtKB.
 GO:0030331; F:estrogen receptor binding; IDA:UniProtKB.
 GO:0042974; F:retinoic acid receptor binding; IDA:UniProtKB.
 GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; IDA:UniProtKB.
 GO:0048387; P:negative regulation of retinoic acid receptor signaling pathway; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:0010606; P:positive regulation of cytoplasmic mRNA processing body assembly; IDA:UniProtKB.
 GO:1900153; P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IMP:UniProtKB.
 GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; IMP:UniProtKB.
 GO:2000036; P:regulation of stem cell maintenance; IMP:UniProtKB.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR007196; CCR4-Not_Not1_C.
 IPR024557; CCR4-Not_Not1su_DUF3819. 
Pfam
 PF12842; DUF3819
 PF04054; Not1 
SMART
  
PROSITE
  
PRINTS