CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037384
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Methyl-CpG-binding domain protein 4 
Protein Synonyms/Alias
  
Gene Name
 MBD4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
56DEEQMMIKRSSECNPubiquitination[1]
117FISPQGLKFRSKSSLubiquitination[2]
397RKDFTGEKIFQEDTIubiquitination[1]
484VLWKFLEKYPSAEVAubiquitination[1, 2, 3]
504RDVSELLKPLGLYDLubiquitination[1]
526FSDEYLTKQWKYPIEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 572 AA 
Protein Sequence
MGTTGLESLS LGDRGAAPTV TSSERLVPDP PNDLRKEDVA MELERVGEDE EQMMIKRSSE 60
CNPLLQEPIA SAQFGATAGT ECRKSVPCGW ERVVKQRLFG KTAGRFDVYF ISPQGLKFRS 120
KSSLANYLHK NGETSLKPED FDFTVLSKRG IKSRYKDCSM AALTSHLQNQ SNNSNWNLRT 180
RSKCKKDVFM PPSSSSELQE SRGLSNFTST HLLLKEDEGV DDVNFRKVRK PKGKVTILKG 240
IPIKKTKKGC RKSCSGFVQS DSKRESVCNK ADAESEPVAQ KSQLDRTVCI SDAGACGETL 300
SVTSEENSLV KKKERSLSSG SNFCSEQKTS GIINKFCSAK DSEHNEKYED TFLESEEIGT 360
KVEVVERKEH LHTDILKRGS EMDNNCSPTR KDFTGEKIFQ EDTIPRTQIE RRKTSLYFSS 420
KYNKEALSPP RRKAFKKWTP PRSPFNLVQE TLFHDPWKLL IATIFLNRTS GKMAIPVLWK 480
FLEKYPSAEV ARTADWRDVS ELLKPLGLYD LRAKTIVKFS DEYLTKQWKY PIELHGIGKY 540
GNDSYRIFCV NEWKQVRLTP IHNSAHLVSE AK 572 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0006284; P:base-excision repair; IEA:InterPro. 
Interpro
 IPR016177; DNA-bd_integrase-typ.
 IPR011257; DNA_glycosylase.
 IPR003265; HhH-GPD_domain.
 IPR017352; Me_CpG-bd_MBD4.
 IPR001739; Methyl_CpG_DNA-bd. 
Pfam
 PF00730; HhH-GPD
 PF01429; MBD 
SMART
 SM00391; MBD 
PROSITE
 PS50982; MBD 
PRINTS