CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021645
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial 
Protein Synonyms/Alias
 MCCase subunit beta; 3-methylcrotonyl-CoA carboxylase 2; 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta 
Gene Name
 MCCC2 
Gene Synonyms/Alias
 MCCB 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
382VLFSESAKKGTHFVQubiquitination[1]
383LFSESAKKGTHFVQLubiquitination[1]
420YEAEGIAKDGAKMVAubiquitination[1, 2]
487NVLATITKDQRAREGubiquitination[1]
495DQRAREGKQFSSADEubiquitination[2]
506SADEAALKEPIIKKFubiquitination[2]
512LKEPIIKKFEEEGNPubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3- methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism. 
Sequence Annotation
 DOMAIN 55 557 Carboxyltransferase.
 REGION 343 372 Acyl-CoA binding (Potential).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Direct protein sequencing; Disease mutation; Ligase; Mitochondrion; Nucleotide-binding; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 563 AA 
Protein Sequence
MWAVLRLALR PCARASPAGP RAYHGDSVAS LGTQPDLGSA LYQENYKQMK ALVNQLHERV 60
EHIKLGGGEK ARALHISRGK LLPRERIDNL IDPGSPFLEL SQFAGYQLYD NEEVPGGGII 120
TGIGRVSGVE CMIIANDATV KGGAYYPVTV KKQLRAQEIA MQNRLPCIYL VDSGGAYLPR 180
QADVFPDRDH FGRTFYNQAI MSSKNIAQIA VVMGSCTAGG AYVPAMADEN IIVRKQGTIF 240
LAGPPLVKAA TGEEVSAEDL GGADLHCRKS GVSDHWALDD HHALHLTRKV VRNLNYQKKL 300
DVTIEPSEEP LFPADELYGI VGANLKRSFD VREVIARIVD GSRFTEFKAF YGDTLVTGFA 360
RIFGYPVGIV GNNGVLFSES AKKGTHFVQL CCQRNIPLLF LQNITGFMVG REYEAEGIAK 420
DGAKMVAAVA CAQVPKITLI IGGSYGAGNY GMCGRAYSPR FLYIWPNARI SVMGGEQAAN 480
VLATITKDQR AREGKQFSSA DEAALKEPII KKFEEEGNPY YSSARVWDDG IIDPADTRLV 540
LGLSFSAALN APIEKTDFGI FRM 563 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004485; F:methylcrotonoyl-CoA carboxylase activity; IDA:UniProtKB.
 GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
 GO:0015936; P:coenzyme A metabolic process; IEA:Compara.
 GO:0006552; P:leucine catabolic process; TAS:UniProtKB. 
Interpro
 IPR000022; Carboxyl_trans.
 IPR011763; COA_CT_C.
 IPR011762; COA_CT_N. 
Pfam
 PF01039; Carboxyl_trans 
SMART
  
PROSITE
 PS50989; COA_CT_CTER
 PS50980; COA_CT_NTER 
PRINTS