CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031956
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Centrosomal protein of 70 kDa 
Protein Synonyms/Alias
 cDNA FLJ60498, highly similar to Centrosomal protein of 70 kDa 
Gene Name
 CEP70 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50RVFAYLCKRVPHTVLubiquitination[1]
104LDASPTYKGLLMSLQubiquitination[1]
115MSLQNQLKESKSKIDubiquitination[1]
120QLKESKSKIDALSSEubiquitination[1]
128IDALSSEKLNLQKDLubiquitination[1, 2]
133SEKLNLQKDLETRPTubiquitination[1]
148QHELRLYKQQVKKLEubiquitination[1]
163KALKKNVKLQELINHubiquitination[1]
171LQELINHKKAEDTEKubiquitination[1, 2]
172QELINHKKAEDTEKKubiquitination[1]
218RAPVIIYKQTKGGVQubiquitination[1, 3]
221VIIYKQTKGGVQNFNubiquitination[2]
275LVPWLNLKKQDENEGubiquitination[4]
276VPWLNLKKQDENEGIubiquitination[1]
441VPAVKKLKVLSY***ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 445 AA 
Protein Sequence
MTEVKCQHYK KKRTEQEETI ASLQMEVCRL KKEEEDRIVT QNRVFAYLCK RVPHTVLDRQ 60
LLCLIDYYES KIRKIHTQRQ YKEDESQSEE ENDYRNLDAS PTYKGLLMSL QNQLKESKSK 120
IDALSSEKLN LQKDLETRPT QHELRLYKQQ VKKLEKALKK NVKLQELINH KKAEDTEKKD 180
EPSKYNQQQA LIDQRYFQVL CSINSIIHNP RAPVIIYKQT KGGVQNFNKD LVQDCGFEHL 240
VPVIEMWADQ LTSLKDLYKS LKTLSAELVP WLNLKKQDEN EGIKVEDLLF IVDTMLEEVE 300
NKEKDSNMPH FQTLQAIVSH FQKLFDVPSL NGVYPRMNEV YTRLGEMNNA VRNLQELLEL 360
DSSSSLCVLV STVGKLCRLI NEDVNEQVMQ VLGPEDLQSI IYKLEEHEEF FPAFQAFTND 420
LLEILEIDDL DAIVPAVKKL KVLSY 445 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0031965; C:nuclear membrane; IDA:HPA. 
Interpro
 IPR013026; TPR-contain_dom.
 IPR019734; TPR_repeat. 
Pfam
  
SMART
  
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS