UniProt Accession

 TF3C4_HUMAN; Q9UKN8; Q5VZJ7 

Genbank Protein ID

 AF142328; AL160165; CH471090; BC011619; BC094774; BC104755; BC112245 

Genbank Nucleotide ID

 AAF05087.1; CAH70534.1; EAW88011.1; AAH11619.1; AAH94774.1; AAI04756.1; AAI12246.1 

Protein Name

 General transcription factor 3C polypeptide 4 

Protein Synonyms/Alias

 TF3C-delta; Transcription factor IIIC 90 kDa subunit; TFIIIC 90 kDa subunit; TFIIIC90; Transcription factor IIIC subunit delta 

Gene Name

 GTF3C4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
33	GGGEAGGKEPAADAA	ubiquitination	[1, 2]
59	TRREPAVKLQYAVSG	ubiquitination	[2, 3]
119	PLNSCLLKVGSKTEV	ubiquitination	[3]
123	CLLKVGSKTEVAECK	ubiquitination	[3]
137	KEKFAASKDPTVSQT	ubiquitination	[1, 3]
156	RVFNPEGKALPPMRG	ubiquitination	[3]
165	LPPMRGFKYTSWSPM	ubiquitination	[3]
225	ETSYRLSKNEAPEGN	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
348	KILPVNLKAVKGYFT	ubiquitination	[9, 10, 11]
351	PVNLKAVKGYFTLRQ	ubiquitination	[3]
364	RQPVILWKEMDQLPV	ubiquitination	[3, 10, 11]
446	YTCSSDGKVRQLIPI	ubiquitination	[9]
460	IFTDVALKFEHQLIK	ubiquitination	[3]
467	KFEHQLIKLSDVFGS	ubiquitination	[8, 10, 11]
598	TPSEALWKPTHEDSK	ubiquitination	[1]
629	QEEGTSSKQVVKQGL	ubiquitination	[1, 2, 3]
633	TSSKQVVKQGLQERS	ubiquitination	[3]
641	QGLQERSKEGDVEEP	ubiquitination	[3]
760	ILPFTDRKQAVCSNG	ubiquitination	[3, 9]
807	PEDPDWIKRLLQSPC	ubiquitination	[1, 2, 3, 4, 5, 9]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [10] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [11] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box. 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 611 611 Phosphoserine.  

Keyword

 Acetylation; Acyltransferase; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 822 AA 

Protein Sequence

Gene Ontology

 GO:0000127; C:transcription factor TFIIIC complex; IDA:HGNC.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008047; F:enzyme activator activity; TAS:ProtInc.
 GO:0004402; F:histone acetyltransferase activity; TAS:ProtInc.
 GO:0042791; P:5S class rRNA transcription from RNA polymerase III type 1 promoter; IC:HGNC.
 GO:0006384; P:transcription initiation from RNA polymerase III promoter; TAS:ProtInc.
 GO:0042797; P:tRNA transcription from RNA polymerase III promoter; IC:HGNC. 

Interpro

 IPR024761; TFIIIC_delta_N.
 IPR024764; TFIIIC_Znf. 

Pfam

 PF12657; TFIIIC_delta
 PF12660; zf-TFIIIC 

SMART

  

PROSITE

  

PRINTS