CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022663
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Unconventional myosin-Va 
Protein Synonyms/Alias
 Dilute myosin heavy chain, non-muscle 
Gene Name
 Myo5a 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
210MESIGNAKTTRNDNSubiquitination[1]
979QLSEEEAKVATGRVLubiquitination[1]
1277RSQLVSQKEAIQPKDubiquitination[1]
1394DLMEQLEKQDKTVRKacetylation[2]
1469KNLILELKPRGVAVNacetylation[2]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation. 
Sequence Annotation
 DOMAIN 1 765 Myosin head-like.
 DOMAIN 766 788 IQ 1.
 DOMAIN 789 813 IQ 2.
 DOMAIN 814 836 IQ 3.
 DOMAIN 837 861 IQ 4.
 DOMAIN 862 884 IQ 5.
 DOMAIN 885 914 IQ 6.
 DOMAIN 1507 1783 Dilute.
 NP_BIND 163 170 ATP (Potential).
 REGION 643 665 Actin-binding (Potential).
 MOD_RES 600 600 Phosphoserine (By similarity).
 MOD_RES 1032 1032 Phosphothreonine.
 MOD_RES 1425 1425 Phosphoserine (By similarity).
 MOD_RES 1733 1733 Phosphothreonine (Potential).  
Keyword
 Actin-binding; ATP-binding; Calmodulin-binding; Coiled coil; Complete proteome; Motor protein; Myosin; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1828 AA 
Protein Sequence
MAASELYTKF ARVWIPDPEE VWKSAELLKD YKPGDKVLLL HLEEGKDLEY RLDPKTSELP 60
HLRNPDILVG ENDLTALSYL HEPAVLHNLR VRFIDSKLIY TYCGIVLVAI NPYEQLPIYG 120
EDIINAYSGQ NMGDMDPHIF AVAEEAYKQM ARDERNQSII VSGESGAGKT VSAKYAMRYF 180
ATVSGSASEA NVEEKVLASN PIMESIGNAK TTRNDNSSRF GKYIEIGFDK RYRIIGANMR 240
TYLLEKSRVV FQAEEERNYH IFYQLCASAK LPEFKMLRLG NADSFHYTKQ GGSPMIEGVD 300
DAKEMAHTRQ ACTLLGISES YQMGIFRILA GILHLGNVGF ASRDSDSCTI PPKHEPLIIF 360
CDLMGVDYEE MCHWLCHRKL ATATETYIKP ISKLQATNAR DALAKHIYAK LFNWIVGHVN 420
QALHSAVKQH SFIGVLDIYG FETFEINSFE QFCINYANEK LQQQFNMHVF KLEQEEYMKE 480
QIPWTLIDFY DNQPCINLIE SKLGILDLLD EECKMPKGTD DTWAQKLYNT HLNKCALFEK 540
PRMSNKAFII KHFADKVEYQ CEGFLEKNKD TVFEEQIKVL KSSKFKMLPE LFQDDEKAIS 600
PTSATSSGRT PLTRVPVKPT KGRPGQTAKE HKKTVGLQFR NSLHLLMETL NATTPHYVRC 660
IKPNDFKFPF TFDEKRAVQQ LRACGVLETI RISAAGFPSR WTYQEFFSRY RVLMKQKDVL 720
GDRKQTCQNV LEKLILDKDK YQFGKTKIFF RAGQVAYLEK LRADKLRAAC IRIQKTIRGW 780
LLRKRYLCMQ RAAITVQRYV RGYQARCYAK FLRRTKAATT IQKYWRMYVV RRKYKIRRAA 840
TIVLQSYLRG YLARNRYRKI LREHKAVIIQ KRVRGWLART HYKRTMKAII YLQCCFRRMM 900
AKRELKKLKI EARSVERYKK LHIGMENKIM QLQRKVDEQN KDYKCLMEKL TNLEGVYNSE 960
TEKLRNDVER LQLSEEEAKV ATGRVLSLQE EIAKLRKDLE QTRSEKKSIE ERADKYKQET 1020
EQLVSNLKEE NTLLKQEKET LNHLMVEQAK EMTETMERKL VEETKQLELD LNDERLRYQN 1080
LLNEFSRLEE RYDDLKEEMT LMLNVPKPGH KRTDSTHSSN ESEYTFSSEF AETEDIAPRT 1140
EEPTEKKVPL DMSLFLKLQK RVTELGQEKQ LMQDELDRKE EQVLRSKAKG GERPQIRGAE 1200
LGYESLKRQE LESENKKLKN ELNELRKALS EKSAPEVNAP GAPAYRVLME QLTAVSEELD 1260
VRKEEVLILR SQLVSQKEAI QPKDDKNTMT DSTILLEDVQ KMKDKGEIAQ AYIGLKETNR 1320
LLESQLQSQK RSHENEAEAL RGEIQSLKEE NNRQQQLLAQ NLQLPPEARI EASLQHEITR 1380
LTNENLDLME QLEKQDKTVR KLKKQLKVFA KKIGELEVGQ MENISPGQII DEPIRPVNIP 1440
RKGKDFQGML EYKREDEQKL VKNLILELKP RGVAVNLISG LPAYILFMCV RHADYLDDDQ 1500
KVRSLLTSTI NSIKKVLKKR GDDFETVSFW LSNTCRFLHC LKQYSGEEGF MKHNTSRQNE 1560
HCLTNFDLAE YRQVLSDLAI QIYQQLVRVL ENILQPMIVS GMLEHETIQG VSGVKPTGLR 1620
KRTSSIADEG TYTLDSILRQ LNSFHSVMCQ HGMDPELIKQ VVKQMFYIVG AITLNNLLLR 1680
KDMCSWSKGM QIRYNVSQLE EWLRDKNLMN SGAKETLEPL IQAAQLLQVK KKTDDDAEAI 1740
CSMCNALTTA QIVKVLNLYT PVNEFEERVS VSFIRTIQVR LRDRKDSPQL LMDAKHIFPV 1800
TFPFNPSSLA LETIQIPASL GLGFIARV 1828 
Gene Ontology
 GO:0042642; C:actomyosin, myosin complex part; IDA:RGD.
 GO:0030424; C:axon; IDA:RGD.
 GO:0032593; C:insulin-responsive compartment; ISS:UniProtKB.
 GO:0043025; C:neuronal cell body; IDA:RGD.
 GO:0008021; C:synaptic vesicle; IDA:RGD.
 GO:0005524; F:ATP binding; IDA:RGD.
 GO:0000146; F:microfilament motor activity; IDA:RGD.
 GO:0046982; F:protein heterodimerization activity; IDA:RGD.
 GO:0032869; P:cellular response to insulin stimulus; ISS:UniProtKB.
 GO:0043473; P:pigmentation; IMP:MGI.
 GO:0072659; P:protein localization to plasma membrane; ISS:UniProtKB.
 GO:0017157; P:regulation of exocytosis; IMP:RGD.
 GO:0032252; P:secretory granule localization; IDA:RGD.
 GO:0030050; P:vesicle transport along actin filament; IMP:RGD.
 GO:0016192; P:vesicle-mediated transport; ISS:UniProtKB. 
Interpro
 IPR018444; Dil_domain.
 IPR002710; Dilute.
 IPR000048; IQ_motif_EF-hand-BS.
 IPR001609; Myosin_head_motor_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF01843; DIL
 PF00612; IQ
 PF00063; Myosin_head 
SMART
 SM00015; IQ
 SM00242; MYSc 
PROSITE
 PS51126; DILUTE
 PS50096; IQ 
PRINTS
 PR00193; MYOSINHEAVY.