UniProt Accession

 TCF25_HUMAN; Q9BQ70; Q2MK75; Q9UPV3 

Genbank Protein ID

 DQ321703; AF322111; BC000959; BC009349; BC011884; AB028972 

Genbank Nucleotide ID

 ABC55265.1; AAG50276.1; AAH00959.1; AAH09349.1; AAH11884.1; BAA83001.1 

Protein Name

 Transcription factor 25 

Protein Synonyms/Alias

 TCF-25; Nuclear localized protein 1 

Gene Name

 TCF25 

Gene Synonyms/Alias

 KIAA1049; NULP1; FKSG26 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
105	QRGNTESKTDGDDTE	ubiquitination	[1]
124	EQSHASGKLRKKKKK	ubiquitination	[1]
136	KKKQKNKKSSTGEAS	ubiquitination	[1]
175	PAPLSSRKHVLYVEH	ubiquitination	[2]
192	LNPDTELKRYFGARA	ubiquitination	[1, 2]
215	QRQRVYPKCTWLTTP	ubiquitination	[2, 3, 4, 5]
223	CTWLTTPKSTWPRYS	ubiquitination	[2]
231	STWPRYSKPGLSMRL	ubiquitination	[1, 2, 3, 4, 5]
243	MRLLESKKGLSFFAF	ubiquitination	[2]
356	KQMSFLEKRGCPRTA	ubiquitination	[1]
545	EACENRRKVLYQRAP	ubiquitination	[2]
565	HVILSEIKEAVAALP	ubiquitination	[6]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development. 

Sequence Annotation

 MOD_RES 602 602 Phosphoserine.  

Keyword

 Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 676 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0007507; P:heart development; NAS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR006994; TCF25.
 IPR011990; TPR-like_helical. 

Pfam

 PF04910; Tcf25 

SMART

  

PROSITE

  

PRINTS