UniProt Accession

 B3KQ59_HUMAN; B3KQ59; E7ETE5 

Genbank Protein ID

 AC008687; AK057498; CH471177 

Genbank Nucleotide ID

 BAG51921.1; EAW52426.1 

Protein Name

 RuvB-like 2 

Protein Synonyms/Alias

 RuvB-like 2 (E. coli), isoform CRA_a; cDNA FLJ32936 fis, clone TESTI2007533, highly similar to RuvB-like 2 (EC 3.6.1.-) 

Gene Name

 RUVBL2 

Gene Synonyms/Alias

 hCG_16214 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
115	GTGSKVGKLTLKTTE	ubiquitination	[1]
119	KVGKLTLKTTEMETI	ubiquitination	[1, 2, 3, 4]
132	TIYDLGTKMIESLTK	ubiquitination	[1]
139	KMIESLTKDKVQAGD	ubiquitination	[1, 2]
141	IESLTKDKVQAGDVI	ubiquitination	[1, 2, 5]
152	GDVITIDKATGKISK	ubiquitination	[1, 2, 5, 6]
178	DAMGSQTKFVQCPDG	ubiquitination	[1, 3, 4, 5, 7]
189	CPDGELQKRKEVVHT	ubiquitination	[1, 2, 3, 4, 5]
191	DGELQKRKEVVHTVS	ubiquitination	[1]
224	SGDTGEIKSEVREQI	ubiquitination	[1, 2, 3, 7]
234	VREQINAKVAEWREE	ubiquitination	[1, 2, 3, 5, 7]
320	STTPYSEKDTKQILR	acetylation	[5, 8]
320	STTPYSEKDTKQILR	ubiquitination	[1, 2, 3, 4, 5, 7]
323	PYSEKDTKQILRIRC	ubiquitination	[1, 2, 5]
372	SLVCRKRKGTEVQVD	ubiquitination	[1, 2, 5]
382	EVQVDDIKRVYSLFL	acetylation	[8]
382	EVQVDDIKRVYSLFL	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 9]
399	SRSTQYMKEYQDAFL	ubiquitination	[1, 2, 3, 4, 7]
411	AFLFNELKGETMDTS	ubiquitination	[1, 2, 3, 4, 7]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

  

Sequence Annotation

  

Keyword

 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 418 AA 

Protein Sequence

Gene Ontology

 GO:0031011; C:Ino80 complex; IEA:InterPro.
 GO:0030529; C:ribonucleoprotein complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0043141; F:ATP-dependent 5'-3' DNA helicase activity; IEA:InterPro.
 GO:0003684; F:damaged DNA binding; IEA:InterPro.
 GO:0032508; P:DNA duplex unwinding; IEA:GOC.
 GO:0006281; P:DNA repair; IEA:InterPro. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR004504; DNA_repair_RadA.
 IPR012340; NA-bd_OB-fold.
 IPR027417; P-loop_NTPase.
 IPR027238; RuvB-like.
 IPR010339; TIP49_C. 

Pfam

 PF06068; TIP49 

SMART

 SM00382; AAA 

PROSITE

  

PRINTS

 PR01874; DNAREPAIRADA.