UniProt Accession

 GSTA3_MOUSE; P30115; Q544Y6 

Genbank Protein ID

 X65021; M73483; AK014076; AK149543; CH466536; CH466536; BC147272; BC147273 

Genbank Nucleotide ID

 CAA46155.1; AAA37751.1; BAB29143.1; BAE28948.1; EDL14389.1; EDL14390.1; AAI47273.1; AAI47274.1 

Protein Name

 Glutathione S-transferase A3 

Protein Synonyms/Alias

 GST class-alpha member 3; Glutathione S-transferase Ya3; Glutathione S-transferase Yc 

Gene Name

 Gsta3 

Gene Synonyms/Alias

 Gstyc 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
4	****MAGKPVLHYFD	acetylation	[1, 2, 3, 4]
4	****MAGKPVLHYFD	succinylation	[4]
4	****MAGKPVLHYFD	ubiquitination	[5]
64	MVEIDGMKLVQTKAI	acetylation	[2]
64	MVEIDGMKLVQTKAI	ubiquitination	[5]
69	GMKLVQTKAILNYIA	ubiquitination	[5]
78	ILNYIASKYNLYGKD	acetylation	[1, 2]
78	ILNYIASKYNLYGKD	ubiquitination	[5]
84	SKYNLYGKDMKERAI	acetylation	[2, 3, 6, 7]
84	SKYNLYGKDMKERAI	ubiquitination	[5]
123	EKEASLAKIKEQTRN	acetylation	[7]
138	RYFPAFEKVLKSHGQ	acetylation	[1, 3, 4, 7]
138	RYFPAFEKVLKSHGQ	succinylation	[4]
138	RYFPAFEKVLKSHGQ	ubiquitination	[5]
141	PAFEKVLKSHGQDYL	acetylation	[3]
141	PAFEKVLKSHGQDYL	ubiquitination	[5]
195	VSNLPTVKKFLQPGS	acetylation	[2, 4, 7, 8]
195	VSNLPTVKKFLQPGS	succinylation	[4]
195	VSNLPTVKKFLQPGS	ubiquitination	[5]
196	SNLPTVKKFLQPGSQ	acetylation	[2]
196	SNLPTVKKFLQPGSQ	ubiquitination	[5]
211	RKPFDDAKCVESAKK	acetylation	[2]
211	RKPFDDAKCVESAKK	ubiquitination	[5]

Reference

 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [7] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [8] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 

Functional Description

 Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This GST has a high catalytic activity for aflatoxin B1-8,9 epoxide. 

Sequence Annotation

 DOMAIN 3 83 GST N-terminal.
 DOMAIN 85 207 GST C-terminal.
 REGION 54 55 Glutathione binding (By similarity).
 REGION 67 68 Glutathione binding (By similarity).
 BINDING 9 9 Glutathione (By similarity).
 BINDING 45 45 Glutathione (By similarity).
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 221 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004364; F:glutathione transferase activity; ISS:UniProtKB.
 GO:0006749; P:glutathione metabolic process; ISS:UniProtKB.
 GO:0001657; P:ureteric bud development; IEP:UniProtKB. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR004045; Glutathione_S-Trfase_N.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR003080; GST_alpha.
 IPR004046; GST_C.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF00043; GST_C
 PF02798; GST_N 

SMART

  

PROSITE

 PS50405; GST_CTER
 PS50404; GST_NTER 

PRINTS

 PR01266; GSTRNSFRASEA.