CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011964
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vesicle transport protein SEC20 
Protein Synonyms/Alias
 BCL2/adenovirus E1B 19 kDa protein-interacting protein 1; Transformation-related gene 8 protein; TRG-8 
Gene Name
 BNIP1 
Gene Synonyms/Alias
 NIP1; SEC20L; TRG8 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
46ALTELNTKVKEKFQQacetylation[1]
48TELNTKVKEKFQQLRacetylation[1]
48TELNTKVKEKFQQLRmethylation[2]
48TELNTKVKEKFQQLRubiquitination[3]
50LNTKVKEKFQQLRHRmethylation[2]
111IAIDNLEKAELLQGGubiquitination[3, 4, 5, 6]
128LRQRKTTKESLAQTSubiquitination[3, 4, 5, 6]
178LDANEEFKSMSGTIQubiquitination[3, 4, 6]
221ATVLYIVKKRLFPFLubiquitination[4]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death. May be involved in mitochondrial autophagy. 
Sequence Annotation
  
Keyword
 Alternative splicing; Apoptosis; Autophagy; Coiled coil; Complete proteome; Endoplasmic reticulum; ER-Golgi transport; Membrane; Mitochondrion; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 228 AA 
Protein Sequence
MAAPQDVHVR ICNQEIVKFD LEVKALIQDI RDCSGPLSAL TELNTKVKEK FQQLRHRIQD 60
LEQLAKEQDK ESEKQLLLQE VENHKKQMLS NQASWRKANL TCKIAIDNLE KAELLQGGDL 120
LRQRKTTKES LAQTSSTITE SLMGISRMMA QQVQQSEEAM QSLVTSSRTI LDANEEFKSM 180
SGTIQLGRKL ITKYNRRELT DKLLIFLALA LFLATVLYIV KKRLFPFL 228 
Gene Ontology
 GO:0030176; C:integral to endoplasmic reticulum membrane; TAS:HGNC.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005635; C:nuclear envelope; IDA:UniProtKB.
 GO:0031201; C:SNARE complex; IDA:HGNC.
 GO:0006915; P:apoptotic process; IPI:MGI.
 GO:0006914; P:autophagy; IEA:UniProtKB-KW.
 GO:0016320; P:endoplasmic reticulum membrane fusion; IMP:HGNC.
 GO:0007029; P:endoplasmic reticulum organization; IMP:HGNC.
 GO:0006917; P:induction of apoptosis; IMP:HGNC.
 GO:0043066; P:negative regulation of apoptotic process; TAS:UniProtKB.
 GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW. 
Interpro
 IPR005606; Sec20. 
Pfam
 PF03908; Sec20 
SMART
  
PROSITE
  
PRINTS