CPLM-017475

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-017475

UniProt Accession

AMPO_HUMAN; Q8N6M6; Q5T9B1; Q5T9B3; Q5T9B4; Q8WUL6; Q96M23; Q96SS1

Genbank Protein ID

AJ560639; AK027581; AK057450; AL157936; AL353768; AL354893; AL353768; AL157936; AL354893; AL354893; AL157936; AL353768; AL157936; AL353768; AL354893; AL353768; AL157936; AL354893; AL354893; AL157936; AL353768; AL157936; AL353768; AL353768; AL157936; AL157936; AL353768; AL354893; AL353768; AL157936; AL354893; AL354893; AL157936; AL353768; AL157936; BC020194; BC029616

Genbank Nucleotide ID

CAD90259.1; BAB55210.1; BAB71491.1; CAI13040.1; CAI13040.1; CAI13040.1; CAI41125.1; CAI41125.1; CAI41125.1; CAI41325.1; CAI41325.1; CAI41325.1; CAI13039.1; CAI13039.1; CAI13039.1; CAI41126.1; CAI41126.1; CAI41126.1; CAI41326.1; CAI41326.1; CAI41326.1; CAI13037.1; CAI13037.1; CAI41124.1; CAI41124.1; CAI13041.1; CAI13041.1; CAI13041.1; CAI41127.1; CAI41127.1; CAI41127.1; CAI41327.1; CAI41327.1; CAI41327.1; CAI13038.1; AAH20194.1; AAH29616.1

Protein Name

Aminopeptidase O

Protein Synonyms/Alias

AP-O

Gene Name

AOPEP

Gene Synonyms/Alias

C9orf3; ONPEP

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
55	EDGNRFKKQNSSIEE	ubiquitination	[1]
169	AAVPGLEKFTRSPEL	ubiquitination	[1]
231	TWSLQIRKTGAQTAT	ubiquitination	[1]

Reference

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart.

Sequence Annotation

ACT_SITE 480 480 Proton acceptor (By similarity).
METAL 479 479 Zinc; catalytic (By similarity).
METAL 483 483 Zinc; catalytic (By similarity).
METAL 502 502 Zinc; catalytic (By similarity).

Keyword

Alternative splicing; Aminopeptidase; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Metalloprotease; Polymorphism; Protease; Reference proteome; Zinc.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

819 AA

Protein Sequence

MDIQLDPARD DLPLMANTSH ILVKHYVLDL DVDFESQVIE GTIVLFLEDG NRFKKQNSSI 60
EEACQSESNK ACKFGMPEPC HIPVTNARTF SSEMEYNDFA ICSKGEKDTS DKDGNHDNQE 120
HASGISSSKY CCDTGNHGSE DFLLVLDCCD LSVLKVEEVD VAAVPGLEKF TRSPELTVVS 180
EEFRNQIVRE LVTLPANRWR EQLDYYARCS QAPGCGELLF DTDTWSLQIR KTGAQTATDF 240
PHAIRIWYKT KPEGRSVTWT SDQSGRPCVY TVGSPINNRA LFPCQEPPVA MSTWQATVRA 300
AASFVVLMSG ENSAKPTQLW EECSSWYYYV TMPMPASTFT IAVGCWTEMK METWSSNDLA 360
TERPFSPSEA NFRHVGVCSH MEYPCRFQNA SATTQEIIPH RVFAPVCLTG ACQETLLRLI 420
PPCLSAAHSV LGAHPFSRLD VLIVPANFPS LGMASPHIMF LSQSILTGGN HLCGTRLCHE 480
IAHAWFGLAI GARDWTEEWL SEGFATHLED VFWATAQQLA PYEAREQQEL RACLRWRRLQ 540
DEMQCSPEEM QVLRPSKDKT GHTSDSGASV IKHGLNPEKI FMQVHYLKGY FLLRFLAKRL 600
GDETYFSFLR KFVHTFHGQL ILSQDFLQML LENIPEEKRL ELSVENIYQD WLESSGIPKP 660
LQRERRAGAE CGLARQVRAE VTKWIGVNRR PRKRKRREKE EVFEKLLPDQ LVLLLEHLLE 720
QKTLSPRTLQ SLQRTYHLQD QDAEVRHRWC ELIVKHKFTK AYKSVERFLQ EDQAMGVYLY 780
GELMVSEDAR QQQLARRCFE RTKEQMDRSS AQVVAEMLF 819

Gene Ontology

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0004177; F:aminopeptidase activity; IDA:UniProtKB.
GO:0008237; F:metallopeptidase activity; IDA:UniProtKB.
GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0019370; P:leukotriene biosynthetic process; IEA:InterPro.
GO:0006508; P:proteolysis; IDA:UniProtKB.

Interpro

IPR016024; ARM-type_fold.
IPR001930; Peptidase_M1.
IPR015211; Peptidase_M1_C.
IPR014782; Peptidase_M1_N.

Pfam

PF09127; Leuk-A4-hydro_C
PF01433; Peptidase_M1

SMART

PROSITE

PS00142; ZINC_PROTEASE

PRINTS