UniProt Accession

 SRSF2_MOUSE; Q62093; Q542L3; Q60701 

Genbank Protein ID

 U14648; AF077858; AK086103; AK088042; AK165670; BC005493; X98511 

Genbank Nucleotide ID

 AAA64595.1; AAC71000.1; BAC39610.1; BAC40111.1; BAE38330.1; AAH05493.1; CAA67134.1 

Protein Name

 Serine/arginine-rich splicing factor 2 

Protein Synonyms/Alias

 Protein PR264; Putative myelin regulatory factor 1; MRF-1; Splicing component, 35 kDa; Splicing factor SC35; SC-35; Splicing factor, arginine/serine-rich 2 

Gene Name

 Srsf2 

Gene Synonyms/Alias

 Pr264; Sfrs10; Sfrs2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
17	VEGMTSLKVDNLTYR	ubiquitination	[1]
36	TLRRVFEKYGRVGDV	acetylation	[2]
36	TLRRVFEKYGRVGDV	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA (By similarity). Can bind to the myelin basic protein (MBP) gene MB3 regulatory region and increase transcription of the mbp promoter in cells derived from the CNS. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment (By similarity). 

Sequence Annotation

 DOMAIN 14 92 RRM.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 23 23 Phosphotyrosine (By similarity).
 MOD_RES 26 26 Phosphoserine (By similarity).
 MOD_RES 36 36 N6-acetyllysine (By similarity).
 MOD_RES 52 52 N6-acetyllysine (By similarity).
 MOD_RES 187 187 Phosphoserine.
 MOD_RES 189 189 Phosphoserine.
 MOD_RES 191 191 Phosphoserine.
 MOD_RES 204 204 Phosphoserine (By similarity).
 MOD_RES 206 206 Phosphoserine.
 MOD_RES 208 208 Phosphoserine.
 MOD_RES 212 212 Phosphoserine.
 MOD_RES 220 220 Phosphoserine (By similarity).
 MOD_RES 221 221 Phosphoserine (By similarity).  

Keyword

 Acetylation; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 221 AA 

Protein Sequence

Gene Ontology

 GO:0016607; C:nuclear speck; IDA:MGI.
 GO:0005681; C:spliceosomal complex; IDA:MGI.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS