CPLM-023755

Genbank Nucleotide ID

Ribosome maturation protein SBDS

Protein Synonyms/Alias

Shwachman-Bodian-Diamond syndrome protein

Homo sapiens (Human)

Position	Peptide	Type	References
62	SVFVNVSKGQVAKKE	ubiquitination	[1, 2]
90	ICKQILTKGEVQVSD	ubiquitination	[1, 2, 3]
98	GEVQVSDKERHTQLE	acetylation	[4]
98	GEVQVSDKERHTQLE	ubiquitination	[1, 2, 3]
118	IATIVADKCVNPETK	ubiquitination	[3]
125	KCVNPETKRPYTVIL	ubiquitination	[3]
151	VKTNKSTKQQALEVI	ubiquitination	[3]
159	QQALEVIKQLKEKMK	ubiquitination	[1, 2, 3]
225	REIDELIKKETKGKG	acetylation	[4]
225	REIDELIKKETKGKG	ubiquitination	[5]
248	DVEEGDEKFE*****	ubiquitination	[6]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFTUD1, triggers the GTP- dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation.

MOD_RES 2 2 N-acetylserine.

3D-structure; Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Disease mutation; Nucleus; Reference proteome; Ribosome biogenesis.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IDA:UniProtKB.
GO:0005730; C:nucleolus; IDA:UniProtKB.
GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
GO:0000922; C:spindle pole; IDA:UniProtKB.
GO:0008017; F:microtubule binding; IDA:UniProtKB.
GO:0043022; F:ribosome binding; IDA:UniProtKB.
GO:0019843; F:rRNA binding; IDA:UniProtKB.
GO:0048539; P:bone marrow development; IMP:UniProtKB.
GO:0030282; P:bone mineralization; IMP:UniProtKB.
GO:0008283; P:cell proliferation; IMP:UniProtKB.
GO:0001833; P:inner cell mass cell proliferation; IEA:Compara.
GO:0030595; P:leukocyte chemotaxis; IDA:UniProtKB.
GO:0042256; P:mature ribosome assembly; IDA:UniProtKB.
GO:0043148; P:mitotic spindle stabilization; IDA:UniProtKB.
GO:0042273; P:ribosomal large subunit biogenesis; IBA:RefGenome.
GO:0006364; P:rRNA processing; IMP:UniProtKB.

IPR018978; Ribosome_mat_SBDS_C.
IPR018023; Ribosome_mat_SBDS_CS.
IPR019783; Ribosome_mat_SBDS_N.
IPR002140; Ribosome_maturation_pr_SBDS.

PF01172; SBDS
PF09377; SBDS_C