UniProt Accession

 FBP1L_HUMAN; Q5T0N5; Q5T0N6; Q6B097; Q6P653; Q6R4Q4; Q9NXG1 

Genbank Protein ID

 AY514449; AL109613; AC095034; AL512651; AL109613; AC095034; AL512651; AL109613; AC095034; AL512651; AL512651; AC095034; AL109613; AL512651; AC095034; AL109613; AL512651; AC095034; AL109613; BC062477; BC074891; BC074892; AK000282 

Genbank Nucleotide ID

 AAR98814.1; CAI18952.1; CAI18952.1; CAI18952.1; CAI18953.1; CAI18953.1; CAI18953.1; CAI18954.1; CAI18954.1; CAI18954.1; CAI18979.1; CAI18979.1; CAI18979.1; CAI18980.1; CAI18980.1; CAI18980.1; CAI18981.1; CAI18981.1; CAI18981.1; AAH62477.1; AAH74891.1; AAH74892.1; BAA91051.1 

Protein Name

 Formin-binding protein 1-like 

Protein Synonyms/Alias

 Transducer of Cdc42-dependent actin assembly protein 1; Toca-1 

Gene Name

 FNBP1L 

Gene Synonyms/Alias

 C1orf39; TOCA1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
109	MRYAHDLKTERKMHL	ubiquitination	[1]
121	MHLQEGRKAQQYLDM	ubiquitination	[1]
165	DNDTNATKADVEKAK	ubiquitination	[2]
172	KADVEKAKQQLNLRT	ubiquitination	[1]
186	THMADENKNEYAAQL	ubiquitination	[2]
272	QMVVDSFKSGFEPPG	ubiquitination	[2]
321	TVGKAKGKLWLFGKK	ubiquitination	[3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Essential for autophagy of intracellular bacterial pathogens. 

Sequence Annotation

 DOMAIN 1 65 FCH.
 REPEAT 409 484 REM.
 DOMAIN 538 599 SH3.
 REGION 1 287 F-BAR domain (By similarity).
 REGION 245 535 Interaction with CDC42.
 REGION 522 605 Interaction with DNM1.
 REGION 541 605 Interaction with DAAM1, DIAPH1 and
 REGION 541 597 Interaction with DNM2 and WASL.
 MOD_RES 295 295 Phosphoserine.
 MOD_RES 488 488 Phosphoserine.
 MOD_RES 501 501 Phosphoserine.  

Keyword

 Alternative splicing; Autophagy; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Endocytosis; Lipid-binding; Membrane; Phosphoprotein; Reference proteome; SH3 domain. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 605 AA 

Protein Sequence

Gene Ontology

 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IDA:BHF-UCL.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0006914; P:autophagy; IEA:UniProtKB-KW.
 GO:0072583; P:clathrin-mediated endocytosis; IDA:BHF-UCL.
 GO:0006900; P:membrane budding; IDA:BHF-UCL.
 GO:0010324; P:membrane invagination; IDA:BHF-UCL.
 GO:0097320; P:membrane tubulation; IDA:BHF-UCL.
 GO:0051491; P:positive regulation of filopodium assembly; IDA:BHF-UCL.
 GO:0030050; P:vesicle transport along actin filament; IDA:BHF-UCL. 

Interpro

 IPR001060; FCH_dom.
 IPR001452; SH3_domain. 

Pfam

 PF00611; FCH
 PF00018; SH3_1 

SMART

 SM00055; FCH
 SM00326; SH3 

PROSITE

 PS50133; FCH
 PS50002; SH3 

PRINTS