CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022015
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable ATP-dependent RNA helicase DDX4 
Protein Synonyms/Alias
 DEAD box protein 4; Vasa homolog 
Gene Name
 DDX4 
Gene Synonyms/Alias
 VASA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
212GSERGGYKGLNEEVIubiquitination[1]
224EVITGSGKNSWKSEAubiquitination[1]
432RLMDIIGKEKIGLKQacetylation[2]
530GQFSKREKLVEILRNacetylation[3]
Reference
 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 May play a role in germ cell development. May play a role in sperm motility. 
Sequence Annotation
 DOMAIN 319 502 Helicase ATP-binding.
 DOMAIN 530 675 Helicase C-terminal.
 NP_BIND 332 339 ATP (By similarity).
 MOTIF 288 316 Q motif.
 MOTIF 446 449 DEAD box.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Developmental protein; Helicase; Hydrolase; Nucleotide-binding; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 724 AA 
Protein Sequence
MGDEDWEAEI NPHMSSYVPI FEKDRYSGEN GDNFNRTPAS SSEMDDGPSR RDHFMKSGFA 60
SGRNFGNRDA GECNKRDNTS TMGGFGVGKS FGNRGFSNSR FEDGDSSGFW RESSNDCEDN 120
PTRNRGFSKR GGYRDGNNSE ASGPYRRGGR GSFRGCRGGF GLGSPNNDLD PDECMQRTGG 180
LFGSRRPVLS GTGNGDTSQS RSGSGSERGG YKGLNEEVIT GSGKNSWKSE AEGGESSDTQ 240
GPKVTYIPPP PPEDEDSIFA HYQTGINFDK YDTILVEVSG HDAPPAILTF EEANLCQTLN 300
NNIAKAGYTK LTPVQKYSIP IILAGRDLMA CAQTGSGKTA AFLLPILAHM MHDGITASRF 360
KELQEPECII VAPTRELVNQ IYLEARKFSF GTCVRAVVIY GGTQLGHSIR QIVQGCNILC 420
ATPGRLMDII GKEKIGLKQI KYLVLDEADR MLDMGFGPEM KKLISCPGMP SKEQRQTLMF 480
SATFPEEIQR LAAEFLKSNY LFVAVGQVGG ACRDVQQTVL QVGQFSKREK LVEILRNIGD 540
ERTMVFVETK KKADFIATFL CQEKISTTSI HGDREQRERE QALGDFRFGK CPVLVATSVA 600
ARGLDIENVQ HVINFDLPST IDEYVHRIGR TGRCGNTGRA ISFFDLESDN HLAQPLVKVL 660
TDAQQDVPAW LEEIAFSTYI PGFSGSTRGN VFASVDTRKG KSTLNTAGFS SSQAPNPVDD 720
ESWD 724 
Gene Ontology
 GO:0033391; C:chromatoid body; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0071546; C:pi-body; ISS:UniProtKB.
 GO:0071547; C:piP-body; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0007141; P:male meiosis I; IEA:Compara.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0032880; P:regulation of protein localization; IEA:Compara.
 GO:0030317; P:sperm motility; IMP:UniProtKB.
 GO:0007283; P:spermatogenesis; IEA:Compara. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS